Title:
How sisters get together; Mechanisms of sister chromatid cohesion

Tomoko Nishiyama
Division of Biological Science, Graduate School of Science Nagoya University

Abstract:
 Cohesin-mediated sister chromatid cohesion is essential for maintaining genome integrity. Cohesin is a ring-shaped multi-protein complex, which in tern recruits several associated proteins like Sororin and Wapl. In vertebrates, these associated proteins are required for changing dynamics of cohesin complex on DNA, so that cohesion can be established or resolved according to cell cycle stages. We have recently demonstrated that Sororin is essential for cohesion establishment in S-phase. We provided evidence that Sororin loaded onto chromatin-bound cohesin during S-phase interferes with the function of Wapl, which promotes dissociation of cohesin from DNA. The molecular mechanisms how sister chromatid cohesion is established during DNA replication and how it’s resolved in mitosis will be discussed.

References:
Nishiyama T, Ladurner R, Schmitz J, Kreidl E, Schleiffer A, Bhaskara V, Bando M, Shirahige K, Hyman AA, Mechtler K, Peters JM.
Sororin mediates sister chromatid cohesion by antagonizing Wapl.
Cell. 2010 Nov 24;143(5):737-49.