【Abstract】
Somatic reprogramming, which was first identified in rodents, remains poorly described in non-model and non-mammalian species. In this context, it is therefore of the primed interest to investigate this original process and compare its efficiency in different species.
We first generated chicken and duck reprogrammed cells and demonstrated that the efficient generation of long-term proliferating cells depends on the method of delivery of reprogramming factors and of the addition of NANOG and LIN28 to the canonical OCT4, SOX2, KLF4, and c-MYC gene combination. These avian reprogrammed cells were compared with embryo derived ESCs and shared several key pluripotency-associated markers.
Second, we also generated new stem cell lines from rent animals including bovine, pig and horse with both canonical and new original gene combination of reprogramming factors. Those reprogrammed stem cells were compared with the canonical induced pluripotent stem (iPS) cells derived from the mouse and human models.
Finally, the somatic reprogramming approach was used to generate megabat stem cell lines that were characterized and exhibited a very high susceptibility to specific viruses when compared to the initial primary cells. As bats carry more zoonotic viruses than any other reservoir host, some of them highly pathogenic in humans (rabies, Ebola, SARS, Nipah, etc…), this original new model of stem cells opens new perspectives to study virus-host interactions in particular the cellular response controlling either susceptibility or restriction to virus infection.
【Contact】Hitoshi Niwa, Dept of Pluripotent Stem Cell Biology (Ext. 6620)
共催；熊本大学国際先端研究拠点、トランスオミクス医学研究拠点ネットワーク形成事業
