国立大学法人熊本大学 国際先端研究拠点
“International Research Core for Stem Cell-based Developmental Medicine”





発生医学研究所 腎臓発生分野 西中村 隆一
1. Ipulan LA, Suzuki K, Sakamoto Y, Murashima A, Imai Y, Omori A, Nakagata N, Nishinakamura R, Valasek P, and Yamada G*. Non-myocytic androgen receptor regulates the sexually dimorphic development of the embryonic bulbocavernosus muscle. Endocrinology  155(7):2467-2479, 2014.
2. Kanda S, Tanigawa S, Ohmori T, Taguchi A, Kudo K, Suzuki Y, Sato Y, Hino S, Sander M, Perantoni AO, Sugano S, Nakao M, and Nishinakamura R*. Sall1 maintains nephron progenitors and nascent nephrons by acting as both an activator and a repressor J. Am. Soc. Nephrol. 25(11): 2584-2595, 2014.
3. Nishita M, Qiao S, Miyamoto M, Okinaka Y, Yamada M, Hashimoto R, Iijima K, Otani H, Hartmann C,Nishinakamura R, and Minami Y*. Role of Wnt5a-Ror2 signaling in morphogenesis of the metanephric mesenchyme during ureteric budding. Mol. Cell Biol. 34(16): 3096-3105, 2014.
4. Tanaka SS*and Nishinakamura R. Regulation of male sex determination: genital ridge formation and Sry activation in mice. Cell. Mol. Life Sci. 71(24): 4781-4802, 2014.
5. Recuenco MC, Ohmori T, Tanigawa S, Taguchi A, Fujimura S, Conti MA, Wei Q, Kiyonari H, Abe T, Adelstein RS and Nishinakamura R. Non-muscle myosin II regulates the morphogenesis of metanephric mesenchyme-derived immature nephrons. J. Am. Soc. Nephrol. 26(5): 1081-1091, 2015.
6. Suzuki K, Numata T, Suzuki H, Raga DD, Ipulan LA, Yokoyama C, Matsushita S, Hamada M, Nakagata N, Nishinakamura R, Kume S, Takahashi S, and Yamada G. Sexually dimorphic expression of Mafb regulates masculinization of the embryonic urethral formation. Proc. Natl. Acad. Sci. USA. 111(46): 16407-16412, 2014.
7. Yamaguchi YL, Tanaka SS, Kumagai M, Fujimoto Y, Terabayashi T, Matsui Y, and Nishinakamura R*. Sall4 is essential for mouse primordial germ cell specification by suppressing somatic program genes.Stem Cells 33(1): 289-300, 2015.
8. Taguchi A and Nishinakamura R*. Nephron reconstitution from pluripotent stem cells. Kidney Int.87: 894-900, 2015.
9. Chiga M, Ohmori T, Ohba T, Katabuchi H and Nishinakamura R. Preformed Wolffian duct regulates Müllerian duct elongation independently of canonical Wnt signaling or Lhx1 expression. Int. J. Dev. Biol.58(9): 643-718, 2014.


発生医学研究所 多能性幹細胞分野 粂 昭苑
1. Sakano D, Shiraki N, Kume S. ‘Pancreatic differentiation from murine ES cells’ in “Human Embryonic Stem Cells, 3rd Edition” (Springer’s Protocols On Line series) (Edited by Kursad Turksen), in press
2. Tsuyama T, Shiraki N, Kume S. “Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation”, Human Embryonic Stem Cells, 3rd Edition (Springer’s Protocols On Line series) (Edited by Kursad Turksen), in press
3. Umeda K, Shiraki N, Kume S, Hepatic differentiation from human iPS cells using M15 cells, Methods Mol. Biol. 2014 Nov 23. [Epub ahead of print] in “iPS Cells: Generation Characterization and Differentiation –Methods and Protocols” (Springer’s Protocols On Line series)(Edited by Andras Nagy and Kursad Turksen).
4. Yamazoe T, Shiraki N, Kume S, Hepatic differentiation from murine and human iPS cells using nanofiber scaffolds, Methods Mol. Biol. 2014 Nov 20. Epub. in “ES Cells: Methods and Protocols- 2nd Edition” (Springer’s Protocols On Line series) (Edited by Andras Nagy and Kursad Turksen).
5. Suzuki K, Numada T, Suzuki H, Raga D, Ipulan L A, Yokoyama C, Matsushita S, Hamada M, Nakagata N, Nishinakamura R, Kume S, Takahashi S and Yamada G. Sexually dimorphic expression of MafB regulates masculinization of the embryonic urethral formation. Proc. Nat. Acad. Sci. USA 111, 16407-16412, 2014.
6. Shiraki N, Ogaki S, Kume S*. Profiling of embryonic stem cell differentiation. Rev. Diabet. Stud.11(1):102-14, 2014.
7. Shahjalal HM, Shiraki N, Sakano D, Kikawa H, Ogaki S, Baba H, Kume K., Kume S. Generation of insulin-producing beta-like cells from human iPS cells in a defined and completely Xeno-free culture system. J. Mol. Cell Biol. 6, 394-408, 2014.
8. Nakashima R, Yano T, Ogawa J, Tanaka N, Toda N, Yoshida M, Takano R, Inoue M, Honda T, Kume Sand Matsumoto K. Potentiation of insulin secretion and improvement of glucose intolerance by combining a novel G protein-coupled receptor 40 agonist DS-1558 with glucagon-like peptide-1 receptor agonists. Eur. J. Pharmacol. 737, 194-201, 2014.
9. Shiraki N, Shiraki Y, Tsuyama T, Obata F, Miura M, Nagae G, Aburatani H, Kume K, Endo F, Kume S*.Methionine metabolism regulates maintenance and differentiation of human pluripotent stem cells. Cell Metab. 19, 780-794, 2014.
10. Kikawa K, Sakano D, Shiraki N, Kume K, Endo F, Kume S*. The beneficial effect of insulin treatment on the outcome of islet transplantation in Akita mice. PLoS ONE 9(4): e95451, 2014. doi:10.1371/journal.pone.0095451


発生医学研究所 細胞医学分野 中尾 光善
1. Tokunaga K, Saitoh N, Goldberg I.G, Sakamoto C, Yasuda Y, Yoshida Y, Yamanaka S, and Nakao M. Computational image analysis of colony and nuclear morphology to evaluate human induced pluripotent stem cells. Sci. Rep. 4: 6996, 2014.
2. Kanda S, Ohmori T, Taguchi A, Kudo K, Horiuchi T, Sato Y, Hino S, Suzuki Y, Sander M, Sugano S,Nakao M, and Nishinakamura R. Sall1 co-operates with Six2 to actively maintain nephron progenitors. J. Am. Soc. Nephrol. 25: 2584-2595, 2014.
3. Sugeno N, Hasegawa T, Tanaka N, Fukuda M, Wakabayashi K, Oshima R, Konno M, Miura E, Kikuchi A, Baba T, Anan T, Nakao M, Geisler S, Aoki M, and Takeda A. K63-linked ubiquitination by Nedd4-1 facilitates endosomal sequestration of internalized alpha-synuclein. J. Biol. Chem. 289: 18137-18151, 2014.
4. Baba T, Otake H, Sato T, Miyabayashi K, Shishido Y, Wang C-Y, Shima Y, Kimura H, Yagi M, Ishihara Y, Hino S, Ogawa H, Nakao M, Yamazaki T, Kang D, Ohkawa Y, Suyama M, Chung B-C, and Morohashi K. Glycolytic genes as the targets of a nuclear receptor Ad4BP/SF-1. Nat. Commun. 5: 3634, 2014.
5. Murata A, Baba Y, Watanabe M, Shigaki H, Miyake K, Ishimoto T, Iwatsuki M, Iwagami S, Yoshida N, Oki E, Morita M, Nakao M, and Baba H. IGF2 DMR0 methylation, loss of imprinting, and patient prognosis in esophageal squamous cell carcinoma. Ann. Surg. Oncol. 21: 1166-1174, 2014.
6. Baba Y, Watanabe M, Murata A, Shigaki H, Miyake K, Ishimoto T, Iwatsuki M, Iwagami S, Yoshida N, Oki E, Sakamaki K, Nakao M, and Baba H. LINE-1 hypomethylation, DNA copy number alterations, and CDK6 amplification in esophageal squamous cell carcinoma. Clin. Cancer Res. 20: 1114-1124, 2014.


発生医学研究所 幹細胞誘導分野 江良 択実
1. Fujie Y, Fusaki N, Katayama T, Hamasaki M, Soejima Y, Soga M, Ban H, Hasegawa M, Yamashita S, Kimura S, Suzuki S, Matsuzawa T, Akari H and Era T. New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood. PLoS One. 9 :e113052 (2014).
2.  Huang GJ, Edwards A, Tsai CY, Lee YS, Peng L, Era T, Hirabayashi Y, Tsai CY, Nishikawa S, Iwakura Y, Chen SJ, Flint J. Ectopic cerebellar cell migration causes maldevelopment of Purkinje cells and abnormal motor behaviour in Cxcr4 null mice. PLoS One. 9(2):e86471 (2014)


発生医学研究所 分化制御分野 佐々木 洋
1. Mamada H, Sato T, Ota M, *Sasaki H. (2015) Cell competition in mouse NIH3T3 embryonic fibroblasts is controlled by the activity of Tead family proteins and Myc J. Cell Sci. 128, 790-803.
2. Wicklow E, Blij S, Frum R, Hirate Y, Lang RA, Sasaki H, *Ralston A. (2014) HIPPO pathway members restrict SOX2 to the inner cell mass where it promotes ICM fates in the mouse blastocyst PLoS Genet. 10, e1004618.
3. Rayon T, Menchero S, Nieto A, Xenopoulos P, Crespo M, Cockburn K, Cañon S, Sasaki H,Hadjantonakis AK, de la Pompa JL, Rossant J, *Manzanares M. (2014) Notch and hippo converge on cdx2 to specify the trophectoderm lineage in the mouse blastocyst. Mechanical control of notochord morphogenesis by extra-embryonic tissues in mouse embryos Dev. Cell. 30, 410-422.
4. Imuta Y, Koyama H, Shi D, Eiraku M, Fujimori T, *Sasaki H (2014) Mechanical control of notochord morphogenesis by extra-embryonic tissues in mouse embryos Mech. Dev. 132, 44-58.


視機能病態学分野 谷原 秀信
1. Takihara Y, Inatani M, Ogata-Iwao M, Kawai M, Inoue T, Iwao K, Tanihara H. Trabeculectomy for open-angle glaucoma in phakic eyes vs in pseudophakic eyes after phacoemulsification: a prospective clinical cohort study. JAMA Ophthalmology 132: 69-76, 2014.
2. Inoue T, Kawaji T, Tanihara H. Monocyte chemotactic protein-1 level in the aqueous humor as a prognostic factor for the outcome of trabeculectomy. Clinical & Experimental Ophthalmology 42: 334-341, 2014.
3. Iwao K, Inatani M, Seto T, Takihara Y, Ogata-Iwao M, Okinami S, Tanihara H. Long-term outcomes and prognostic factors for trabeculectomy with mitomycin C in eyes with uveitic glaucoma: a retrospective cohort study. Journal of Glaucoma 23: 88-94, 2014.
4. Kojima S, Inoue T, Kawaji T, Tanihara H. Filtration bleb revision guided by three-dimensional anterior segment optical coherence tomography. Journal of Glaucoma 23: 312-315, 2014.
5. Kojima S, Inatani M, Shobayashi K, Haga, A, Inoue T, Tanihara H. Risk factors for hyphema after trabeculectomy with mitomycin C. Journal of Glaucoma 23: 307-311, 2014.
6. Yamamoto T, Sawada A, Mayama C, Araie M, Ohkubo S, Sugiyama K, Kuwayama Y, The Collaborative Bleb-related Infection Incidence and Treatment Study Group: The 5-year incidence of bleb-related infection and its risk factors after filtering surgeries with adjunctive mitomycin C: collaborative bleb-related infection incidence and treatment study 2. Ophthalmology 121: 1001-1006, 2014.
7. Takahashi E, Inoue T, Fujimoto T, Kojima S, Tanihara H. Epithelial mesenchymal transition-like phenomenon in trabecular meshwork cells. Experimental Eye Research 118: 72-79, 2014.
8. Kojima S, Inoue T, Kawaji T, Tanihara H. Tear fluid signs associated with filtration blebs, as demonstrated by three-dimensional anterior segment optical coherence tomography. Clinical Ophthalmology 8: 767-772, 2014.
9. Kawaji T, Inoue T, Hara R, Eiki D, Ando Y, Tanihara H. Long-term outcomes and complications of trabeculectomy for secondary glaucoma in patients with familial amyloidotic polyneuropathy. PLoS One9: e96324, 2014
10. Kuroda U, Inoue T, Awai-Kasaoka N, Shobayashi K, Kojima S, Tanihara H. Fornix-based versus limbal-based conjunctival flaps in trabeculectomy with mitomycin C in high-risk patients. Clinical Ophthalmology 8: 949-954. 2014.


免疫学分野 阪口 薫雄
1. Kitabatake M, Soma M, Zhang T, Kuwahara K, Fukushima Y, Nojima T, Kitamura D, Sakaguchi N. JNK regulatory molecule G5PR induces IgG autoantibody-producing plasmablasts from peritoneal B1a cells. J Immunol. 2015 194(4):1480-8.
2. Mouri Y, Nishijima H, Kawano H, Hirota F, Sakaguchi N, Morimoto J, Matsumoto M. NF-κB-inducing kinase in thymic stroma establishes central tolerance by orchestrating cross-talk with not only thymocytes but also dendritic cells. J Immunol. 2014 193(9):4356-67.
3. Eid MM, Maeda K, Almofty SA, Singh SK, Shimoda M, Sakaguchi N. GANP regulates the choice of DNA repair pathway by DNA-PKcs interaction in AID-dependent IgV region diversification. J Immunol. 2014 192(12):5529-39..


神経分化学分野 太田 訓正
1. Niimori D, Kawano R, Niimori-Kita K, Ihn H and *Ohta K. Tsukushi is involved in the wound healing by regulating the expression of cytokines and growth factors. J. Cell Communication and Signaling, 8, 173-177 (2014)
2. Song X, Tanaka H, and *Ohta K. Multiple role of Equarin during lens development. Develop. Growth Differ., 56, 199-205 (2014)
3. Riyadh A, Shinmyo Y, Ohta K, and *Tanaka H. Inhibitory effects of draxin on axonal outgrowth and migration of precerebellar neurons. BBRC, 449, 169-174 (2014)


遺伝子機能応用学分野 甲斐広文
1. Suico MA, Fukuda R, Miyakita R, Koyama K, Taura M, Shuto T, Kai H. The transcription factor MEF/Elf4 is dually modulated by p53-MDM2 axis and MEF-MDM2 autoregulatory mechanism. J. Biol. Chem. 2014; 289(38): 26143-54
2. Matsuyama S, Moriuchi M, Suico MA, Yano S, Morino-Koga S, Shuto T, Yamanaka K, Kondo T, Araki E,Kai H. Mild electrical stimulation increases stress resistance and suppresses fat accumulation via activation of LKB1-AMPK signaling pathway in C. elegans. PLoS One. 2014; 9(12):e114690
3. Shuto KU, Kato K, Tasaki Y, Sato M, Sato K, Uchida Y, Sakai H, Ono T, Suico MA, Mitsutake K, Tokutomi N, Kai H, Shuto T. Lipopolysaccharide decreases single immunoglobulin interleukin-1 receptor-related molecule (SIGIRR) expression by suppressing Sp1 via TLR4-p38 pathway in monocytes and neutrophils. J. Biol. Chem.2014; 289(26):18097-109
4. Kondo T, Motoshima H, Igata M, Kawashima J, Matsumura T, Kai H, Araki E.
The role of heat shock response in insulin resistance and diabetes. Diabetes Metab J. 2014; 38(2): 100-106
5.  Kondo T, Ono K, Kitano S, Matsuyama R, Goto R, Suico MA, Kawasaki S, Igata M, Kawashima J, Motoshima H, Matsumura T, Kai H, Araki E. Mild Electrical Stimulation with Heat Shock Reduces Visceral Adiposity and Improves Metabolic Abnormalities in Subjects with Metabolic Syndrome or Type 2 Diabetes: Randomized Crossover Trials. EBioMedicine. 2014; (1):80-89
6.  Aritomi K, Ishitsuka Y, Tomishima Y, Shimizu D, Abe N, Shuto T, Irikura M, Kai H, and Irie T. Evaluation of Three-Dimensional Cultured HepG2 Cells in a Nano Culture Plate System: an In Vitro Human Model of Acetaminophen Hepatatoxicity. J Pharmacol. Sci. 2014; 124: 218-229

7.  Sargent RG, Suzuki S, Gruenert DC. Nuclease-Mediated Double-Strand Break (DSB) Enhancement of Small Fragment Homologous Recombination (SFHR) Gene Modification in Human-Induced Pluripotent Stem Cells (hiPSCs). Methods Mol Biol. 2014; 1114:279-90

8. Kariya R, Taura M, Suzu S, Kai H, Katano H, Okada S. HIV protease inhibitor Lopinavir induces apoptosis of primary effusion lymphoma cells via suppression of NF-κB pathway. Cancer Lett. 2014; 342:52-59
9.  Miyata M, Lee JY, Miyata SS, Wang WY, Xu H, Kai H, Kobayashi KS, Flavell RA & Li JD. Glucocorticoids suppress inflammation via the upregulation of negative regulator IRAK-M. Nat. Commun. 2015; 6:6062(国際共同研究論文)
10.  Taura M, Kudo E, Kariya R, Goto H, Matsuda K, Hattori S, Vaeteewoottacharn K, McDonald F, Suico MA, Shuto T, Kai H, Okada S. COMMD1/Murr1 Reinforces HIV-1 Latent Infection through IκB-α Stabilization. J. Virol. 2014; 89(5):2643-58(国際共同研究論文)


微生物薬学分野 大槻 純男
1. Nishina T, Numata J, Nishina K, Yoshida-Tanaka K, Nitta K, Piao W, Iwata R, Ito S, Kuwahara H, Wada T, Mizusawa H, Yokota T:Chimeric Antisense Oligonucleotide Conjugated to alpha-Tocopherol. Mol Ther Nucleic Acids.4:e220 (2015)
2. Uchida Y, Ohtsuki S, Teriyaki T:Pharmacoproteomics-based reconstruction of in vivo p-glycoprotein function at blood-brain barrier and brain distribution of substrate verapamil in pentylenetetrazole-kindled epilepsy, spontaneous epilepsy, and phenytoin treatment models. Drug Metab Dispos.42:1719-26 (2014)
3. Uchida Y, Wakayama K, Ohtsuki S, Chiba M, Ohe T, Ishii Y, Teriyaki T: Blood-brain barrier pharmacoproteomics-based reconstruction of the in vivo brain distribution of p-glycoprotein substrates in cynomolgus monkeys. J Pharmacol Exp Ther. 350:578-88 (2014)
4. Ohtsuki S, Kawakami H, Inoue T, Nakamura K, Tateno C, Katsukura Y, Obuchi W, Uchida Y, Kamiie J, Horie T, Teriyaki T: Validation of uPA/SCID Mouse with Humanized Liver as a Human Liver Model: Protein Quantification of Transporters, Cytochromes P450, and UDP-Glucuronosyltransferases by LC-MS/MS. Drug Metab Dispos.42:1039-43 (2014)
5. Ito S, Ohtsuki S, Murata S, Katsukura Y, Suzuki H, Funaki M, Tachikawa M, Teriyaki T: Involvement of insulin-degrading enzyme in insulin- and atrial natriuretic peptide-sensitive internalization of amyloid-beta peptide in mouse brain capillary endothelial cells. J Alzheimers Dis.38:185-200 (2014)
6. Chakravarthy B, Ito S, Atkinson T, Gaudet C, Ménard M, Brown L, Whitfield J:Evidence that a synthetic amyloid-ß oligomer-binding peptide (ABP) targets amyloid-ß deposits in transgenic mouse brain and human Alzheimer’s disease brain. Biochem Biophys Res Commun. 445(3):656-60 (2014).
7. Ohtsuki S, Hirayama M, Ito S, Uchida Y, Tachikawa M, Teriyaki T: Quantitative targeted proteomics for understanding the blood-brain barrier: towards pharmacoproteomics. Expert Rev Proteomics.11:303-13 (2014)