【Date】 Dec. 27 (Thu), 2018 16:00～17:00
【Venue】 Conference room, 1st floor,
Institute of Molecular Embryology and Genetics (IMEG),
Kumamoto University
【Abstract】
ES cells and EpiSCs are pluripotent stem cell in culture and their characteristics are distinguished by two terms, naïve and primed. Pluripotency in mouse embryo is a continuum from pre-implantation until the end of gastrulation, however, naïve cells corresponds to pre-implantation epiblast (E3.4-4.5) and that of primed cells are gastrulating cells (around E7.0). There is an uncharacterised phase between E5.0 and E6.5. After implantation, naïve epiblast cells epithelialise and expand without expression of lineage makers until gastrulating primed stage. Epiblast at this stage are able to respond to germ cell induction signals, which is neither naïve or primed epiblast/stem cells not. We deconstruct the pluripotency phases and coin the term “Formative” to this state. Here we discuss features of formative pluripotency and how to capture formative pluripotency as a novel stem cell in culture.
【Contact】Hitoshi Niwa, Dept of Pluripotent Stem Cell Biology (Ext. 6620)
共催；熊本大学国際先端研究拠点、トランスオミクス医学研究拠点ネットワーク形成事業
