国際先端研究拠点
国立大学法人熊本大学 国際先端研究拠点
“幹細胞を用いた臓器再建と次世代医療・創薬を目指す国際的研究教育拠点”
“International Research Core for Stem Cell-based Developmental Medicine”
国際先端研究拠点ホームへ
平成28年度(2016年度)- 令和3年度(2021年度)
発生医学研究所 腎臓発生分野 西中村 隆一
- Kuraoka S, Tanigawa S, Taguchi A, Hotta A, Nakazato H, Osafune K, Kobayashi A, Nishinakamura R. PKD1-dependent renal cytogenesis in human induced pluripotent stem cell-derived ureteric bud/collecting duct organoids.J Am Soc Nephrol 31:2355-2371, 2020.
- Nishinakamura R. Human kidney organoids: progress and remaining challenges.Nat Rev Nephrol 15: 613-624, 2019.
- Tanigawa S, Islam M, Sharmin S, Naganuma H, Yoshimura Y, Haque F, Era T, Nakazato H, Nakanishi K, Sakuma T, Yamamoto T, Kurihara H, Taguchi A, and Nishinakamura R. Organoids from nephrotic disease-derived iPSCs identify impaired NEPHRIN localization and slit diaphragm formation in kidney podocytes. Stem Cell Reports11: 727–740, 2018
- Taguchi A and Nishinakamura R. Higher-order kidney organogenesis from pluripotent stem cells. Cell Stem Cell21: 730-746, 2017.
- Tanigawa S, Taguchi A, Sharma N, Perantoni AO, and Nishinakamura R. Selective in vitro propagation of nephron progenitors from embryos and pluripotent stem cells.Cell Rep 15: 801-813, 2016.
発生医学研究所 多能性幹細胞分野 丹羽 仁史
- Matsuura K, Tani N, Usuki S, Torikai-Nishikawa S, Okano M and Niwa H. MEAF6 is essential for cell proliferation and plays a role in the assembly of KAT7 complexes. Exp Cell Res, 396: 112279, 2020.
- Yamane M, Ohtsuka S, Matsuura K, Nakamura A and Niwa H. Overlapping functions of Kruppel-like factor family members: targetting multiple transcription factors to maintain the naïve pluripotency of mouse embryonic stem cells. Development: 145, 2018.
- Niwa, H. The principles that govern transcription factor network functions in stem cells. Development, 145, 2018.
- Nakai-Futatsugi Y and Niwa H. Zscan4 is activated after telomere shortening in mouse embryonic stem cells. Stem Cell Reports 6: 1-13, 2016.
- Niwa H, Nakamura A, Urata M, Shirae-Kurabayashi M, Shigehiro K, Russell S, and Ohtsuka S. The evolutionally-conserved function of group B1 Sox family members confers the unique role of Sox2 in mouse ES cells. BMC Evolutionary Biology 16: 173, 2016.
発生医学研究所 幹細胞誘導分野 江良 択実
- Odaka H, Numakawa T, Soga M, Kido J, Matsumoto S, Kajihara R, Okumiya T, Tani N, Tanoue Y, Fukuda T, Furuya H, Inoue T and Era T. An iPSC-based neural model of sialidosis uncovers glycolytic impairment-causing presynaptic dysfunction and deregulation of Ca2+dynamics. Neurobiol Dis. 152: 105279, 2021.
- Kajihara R, Numakawa T, Odaka H, Yaginuma Y, Fusaki N, Okumiya T, Furuya H, Inui S and Era T. Novel drug candidates improve ganglioside accumulation and neural dysfunction in GM1 gangliosidosis models with autophagy activation. Stem Cell Reports 14:909-923, 2020.
- Yokouchi Y, Suzuki S, Ohtsuki N, Yamamoto K, Noguchi S, Soejima Y, Goto M, Ishioka K, Nakamura I, Suzuki S, Takenoshita S and Era T. Rapid repair of human disease-specific single-nucleotide variants by One-SHOT genome editing. Sci Rep. 10: 13927, 2020.
- Miwa H and Era T. Tracing the destiny of mesenchymal stem cells from embryo to adult bone marrow and white adipose tissue via Pdgfra expression. Development, Jan 29;145(2)., 2018.
- Khodeer S and Era T. Identifying the Biphasic Role of Calcineurin/NFAT Signaling Enables Replacement of Sox2 in Somatic Cell Reprogramming. Stem Cells 35: 1162-1175, 2017.
発生医学研究所 細胞医学分野 中尾 光善
- Nakao M*, Tanaka H, Koga T. Cellular senescence variation by metabolic and epigenomic remodeling. Trends Cell Biol 30: 919-922, 2020.
- Tanaka H, Igata T, Etoh K, Koga T, Takebayashi S, Nakao M*. The NSD2/WHSC1/MMSET methyltransferase protects cellular senescence-associated epigenomic remodeling. Aging Cell 19: e13173, 2020.
- Abdalla MO, Yamamoto T, Maehara K, Nogami J, Ohkawa Y, Miura H, Poonperm R, Hiratani I, Nakayama H, Nakao M*, Saitoh N*. The Eleanor ncRNAs activate the topological domain of the ESR1 locus to balance against apoptosis. Nat Commun 10: 3778, 2019.
- Nakao M*, Anan K, Araki H, Hino S. Distinct roles of NAD+-Sirt1 and FAD-LSD1 pathways in metabolic response and tissue development. Trends Endocrinol. Metab. 30: 409-412, 2019. Nakao M*, Fujiwara S, Iwase H. Cancer navigation strategy for endocrine therapy-resistant breast tumors. Trends Cancer 4: 404-407, 2018.
- Anan K, Hino S*, Shimizu N, Sakamoto A, Nagaoka K, Takase R, Kohrogi K, Araki H, Hino Y, Usuki S, Oki S, Tanaka H, Nakamura K, Endo F, Nakao M*. LSD1 mediates metabolic reprogramming by glucocorticoids during myogenic differentiation. Nucleic Acids Res. 46: 5441-5454, 2018.
- Tanaka H, Takebayashi S, Sakamoto A, Igata T, Nakatsu Y, Saitoh N, Hino S, Nakao M*. The SETD8/PR-Set7 methyltransferase functions as a barrier to prevent senescence-associated metabolic remodeling. Cell Rep. 18: 2148-2161, 2017.
遺伝子機能応用学分野 甲斐 広文
- Omachi K, Kaseda S, Yokota T, Kamura M, Teramoto K, Kuwazuru J, Kojima H, Nohara H, Koyama K, Ohtsuki S, Misumi S, Takeo T, Nakagata N, Li JD, Shuto T, Suico M.A., Miner J.H., Kai H. Metformin Ameliorates the Severity of Experimental Alport Syndrome. Sci Rep 11:7053, 2021.
- Yamamura T, Horinouchi T, Adachi T, Terakawa M, Takaoka Y, Omachi K, Takasato M, Takaishi K, Shoji T, Onishi Y, Kanazawa Y, Koizumi M, Tomono Y, Sugano A, Shono A, Minamikawa S, Nagano C, Sakakibara N, Ishiko S, Aoto Y, Kamura M, Harita Y, Miura K, Kanda S, Morisada N, Rossanti R, Juan M Ye, Nozu Y, Matsuo M, Kai H, Iijima K, Nozu K. Development of an exon skipping therapy for X-linked Alport syndrome with truncating variants in COL4A5. Nat Commun 11(1):2777, 2020.
- Kamei S, Fujikawa H, Nohara H, Ueno-Shuto K, Maruta K, Nakashima R, Kawakami T, Matsumoto C, Sakaguchi Y, Ono T, Suico MA, Boucher RC, Gruenert DC, Takeo T, Nakagata N, Li JD, Kai H, Shuto T. Zinc Deficiency via a Splice Switch in Zinc Importer ZIP2/SLC39A2 Causes Cystic Fibrosis-Associated MUC5AC Hypersecretion in Airway Epithelial Cells. EBioMedicine. 27: 304-316, 2018.
- Omachi K, Kamura M, Teramoto K, Kojima H, Yokota T, Kaseda S, Kuwazuru J, Fukuda R, Koyama K, Matsuyama S, Motomura K, Shuto T, Suico MA, Kai H. A split-luciferase-based trimer formation assay as a high-throughput screening platform for therapeutics in Alport syndrome.Cell Chem Biol. 25(5): 634-643, 2018.
- Shuto T, Kamei S, Nohara H, Fujikawa H, Tasaki Y, Sugahara T, Ono T, Matsumoto C, Sakaguchi Y, Maruta K, Nakashima R, Kawakami T, Suico MA, Kondo Y, Ishigami A, Takeo T, Tanaka KI, Watanabe H, Nakagata N, Uchimura K, Kitamura K, Li JD, Kai H. Pharmacological and genetic reappraisals of protease and oxidative stress pathways in a mouse model of obstructive lung diseases. Sci Rep 16;6:39305, 2016.
微生物薬学分野 大槻 純男
- Yamaguchi S, Ito S, Masuda T, Couraud PO, Ohtsuki S, Novel cyclic peptides facilitating transcellular blood-brain barrier transport of macromolecules in vitro and in vivo. J Control Release. 321, 744-55, 2020.
- Ito S, Oishi M, Ogata S, Uemura T, Couraud PO, Masuda T, Ohtsuki S, Identification of Cell-Surface Proteins Endocytosed by Human Brain Microvascular Endothelial Cells In Vitro. Pharmaceutics. 12, 2020.
- Ogata S, Ito S, Masuda T, Ohtsuki S, Efficient isolation of brain capillary from a single frozen mouse brain for protein expression analysis. J Cereb Blood Flow Metab. 271678X20941449, 2020.
- Kuno T, Hirayama-Kurogi M, Ito S, Ohtsuki S, Reduction in hepatic secondary bile acids caused by short-term antibiotic-induced dysbiosis decreases mouse serum glucose and triglyceride levels. Sci Rep. 8, 1253, 2018.
- Yamaguchi S, Ito S, Kurogi-Hirayama M, Ohtsuki S, Identification of cyclic peptides for facilitation of transcellular transport of phages across intestinal epithelium in vitro and in vivo. J Control Release. 262, 232-8, 2017.
発生医学研究所 染色体制御分野 石黒 啓一郎
- Horisawa-Takada Y, Kodera C, Takemoto K, Sakashita A, Horisawa K, Maeda R, Shimada R, Usuki S, Fujimura S, Tani N, Matsuura K, Akiyama T, Suzuki A, Niwa H, Tachibana M, Ohba T, Katabuchi H, Namekawa S, Araki K, *Ishiguro K. Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis. Nat Commun12, 3184: (2021)
- Fujiwara Y., Horisawa-Takada Y., Inoue E., Tani , Shibuya H., Fujimura S., Kariyazono R., Sakata T., Ohta K., Araki K., Okada Y., *Ishiguro K. Meiotic cohesins mediate initial loading of HORMAD1 to the chromosomes and coordinate SC formation during meiotic prophase. PLOS Genet 16(9): e1009048. (2020)
- Tanno N., Kuninaka , Fujimura S., Takemoto K., Okamura K., Takeda N., Araki K., Araki M., Saya H. and *Ishiguro K. Phosphorylation of the Anaphase Promoting Complex activator FZR1/ CDH1 is required for Meiosis II entry in mouse male germ cell. Sci Rep 10 10094 (2020)
- Takemoto K., Tani , Takada Y., Fujimura S., Tanno N., Yamane M., Okamura K., Sugimoto M., Araki K., *Ishiguro K. Meiosis-specific C19orf57/4930432K21Rik/ BRME1 modulates localization of RAD51 and DMC1 to DSBs in mouse meiotic recombination. Cell Rep 31, 107686 (2020)
- Ishiguro K, Matsuura K, Tani N, Takeda N, Usuki S, Yamane M, Sugimoto M, Fujimura S, Hosokawa M, Chuma S, Ko S.H.M, Araki K, Niwa H: MEIOSIN directs the switch from mitosis to meiosis in mammalian germ cells. Dev Cell 52(4), 429-445(2020)
発生医学研究所 ゲノム神経学分野 塩田 倫史
- Asamitsu S, Yabuki Y, Ikenoshita S, Kawakubo K, Kawasaki M, Usuki S, Nakayama Y, Adachi K, Kugoh H, Ishii K, Matsuura T, Nanba E, Sugiyama H, Fukunaga K, and Shioda N. CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome. Science Advances 7:eabd9440, 2021.
- Yabuki Y, Matsuo K, Yu M, Xu J, Sakimura K, Shioda N, and Fukunaga K. Cav3.1 T-type calcium channel is critical for cell proliferation and survival in newly generated cells of the adult hippocampus. Acta Physiol. e13613, 2021.
- Shioda N, Imai Y, Yabuki Y, Sugimoto W, Yamaguchi K, Wang Y, Hikida T, Sasaoka T, Mieda M, and Fukunaga K. Dopamine D2L Receptor Deficiency Causes Stress Vulnerability through 5-HT1A Receptor Dysfunction in Serotonergic Neurons. J Neurosci. 39:7551-7563, 2019.
- Asamitsu S, Shioda N, and Sugiyama H. Switching Off Cancer-Causing Telomerase Using Small Molecules. Cell Chem Biol. 26:1045-1047, 2019.
- Asamitsu S, Takeuchi M, Ikenoshita S, Imai Y, Kashiwagi H, and Shioda N. Perspectives for Applying G-Quadruplex Structures in Neurobiology and Neuropharmacology. J. Mol. Sci. 20:2884, 2019.
発生医学研究所 筋発生再生分野 小野 悠介
- Yoshioka K, Nagahisa H, Miura F, Araki H, Kamei Y, Kitajima Y, Seko D, Nogami J, Tsuchiya Y, Okazaki N, Yonekura A, Ohba S, Sumita Y, Chiba K, Ito K, Asahina I, Ogawa Y, Ito T, Ohkawa Y, Ono Y. Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle. Science Advances 2021 Jun 9. 7: eabd7924.
- Yoshioka K, Kitajima Y, Seko D, Tsuchiya Y, Ono Y. The body-region-specificity in murine models of muscle regeneration and atrophy. Acta Physiol. 2021 Jan;231(1):e13553. IF: 311
- Tsuchiya Y, Kitajima Y, Masumoto H, Ono Y. Damaged myofiber-derived metabolic enzymes act as activators of muscle satellite cells. Stem Cell Reports. 2020 Oct 13;15(4):926-940. Seko D, Fujita R, Kitajima Y, Nakamura K, Imai Y, Ono Y. Estrogen receptor β controls muscle growth and regeneration in young female mice. Stem Cell Reports 2020 Sep 8;15:577–586.
- Yoshioka K, Kitajima Y, Okazaki N, Chiba K, Yonekura A, Ono Y. A Modified pre-plating method for high-yield and high-purity muscle stem cell isolation from human/mouse skeletal muscle tissues. Front Cell Dev Biol. 2020 Aug 13;8:793.