国際先端研究拠点
国立大学法人熊本大学 国際先端研究拠点
“幹細胞を用いた臓器再建と次世代医療・創薬を目指す国際的研究教育拠点”
“International Research Core for Stem Cell-based Developmental Medicine”

 

国際先端研究拠点ホームへ

平成28年度(2016年度)

 

発生医学研究所 腎臓発生分野 西中村 隆一

1.Novo, C.L., Tang, C., Ahmed, K., Djuric, U., Fussner, E., Mullin, N.P., Morgan, N.P., Hayre, J., Sienerth, A.R., Elderkin, S., Nishinakamura, R., Chambers, I., Ellis, J., Bazett-Jones, D.P., Rugg-Gunn, P.J. The pluripotency factor Nanog regulates pericentromeric heterochromatin organization in mouse embryonic stem cells. Genes and Development 30(9):1101-1115, 2016

2.Tanigawa, S., Taguchi, A., Sharma, N., Perantoni, A.O., Nishinakamura, R. Selective In Vitro Propagation of Nephron Progenitors Derived from Embryos and Pluripotent Stem Cells. Cell Reports 15(4): 801-813, 2016

3.Buttgereit, A., Lelios, I., Yu, X., Vrohlings, M., Krakoski, N.R., Gautier, E.L., Nishinakamura, R., Becher, B., Greter, M. Sall1 is a transcriptional regulator defining microglia identity and function. Nature Immunology 17(12):1397-1406, 2016

4.Nishinakamura, R. Stem cells and renal development in 2015: Advances in generating and maintaining nephron progenitors. Nature Reviews Nephrology 12(2):67-68, 2016

5.Sasaki, T., Tanaka, Y., Kulkeaw, K., Yumine-Takai, A., Tan, K.S., Nishinakamura, R., Ishida, J., Fukamizu, A., Sugiyama, D. Embryonic Intra-Aortic Clusters Undergo Myeloid Differentiation Mediated by Mesonephros-Derived CSF1 in Mouse. Stem Cell Reviews and Reports 12(5):530-542, 2016

6.Morita, Y., Andersen, P., Hotta, A., Tsukahara, Y., Sasagawa, N., Hayashida, N., Koga, C., Nishikawa, M., Saga, Y., Evans, S.M., Koshiba-Takeuchi, K., Nishinakamura, R., Yoshida, Y., Kwon, C., Takeuchi, J.K. Sall1 transiently marks undifferentiated heart precursors and regulates their fate. Journal of Molecular and Cellular Cardiology 92:158-162, 2016

7.Miller, A., Ralser, M., Kloet, S.L., Loos, R., Nishinakamura, R., Bertone, P., Vermeulen, M., Hendrich, B. Sall4 controls differentiation of pluripotent cells independently of the nucleosome remodelling and deacetylation (NuRD) complex. Development (Cambridge) 143(17):3074-3084, 2016

8.Tanigawa, S., Nishinakamura, R. Expanding nephron progenitors in vitro: a step toward regenerative medicine in nephrology. Kidney International 90(5):925-927, 2016

9.Xiong, J., Zhang, Z., Chen, J., Huang, H., Xu, Y., Ding, X., Zheng, Y., Nishinakamura, R., Xu, G.-L., Wang, H., Chen, S., Gao, S., Zhu, B. Cooperative Action between SALL4A and TET Proteins in Stepwise Oxidation of 5-Methylcytosine. Molecular Cell 64(5):913-925, 2016

 

発生医学研究所 幹細胞誘導分野 江良 択実
1.Motoyama, K., Nishiyama, R., Maeda, Y., Higashi, T., Kawaguchi, Y., Futaki, S., Ishitsuka, Y., Kondo, Y., Irie, T., Era, T., Arima, H. Cholesterol-lowering effect of octaarginine-appended β-cyclodextrin in Npc1-trap-CHO cells. Biological and Pharmaceutical Bulletin 39(11):1823-1829, 2016

2.Masaki, H., Kato-Itoh, M., Takahashi, Y., Umino, A., Sato, H., Ito, K., Yanagida, A., Nishimura, T., Yamaguchi, T., Hirabayashi, M., Era, T., Loh, K.M., Wu, S.M., Weissman, I.L., Nakauchi, H. Inhibition of Apoptosis Overcomes Stage-Related Compatibility Barriers to Chimera Formation in Mouse Embryos. Cell Stem Cell 19(5):587-592, 2016

3.Wang, Z., Nakamura, K., Jinnin, M., Kudo, H., Goto, M., Era, T., Kira, T., Nakashima, T., Fukushima, S., Ihn, H. Establishment and gene expression analysis of disease-derived induced pluripotent stem cells of scleroderma. Journal of Dermatological Science 84(2):186-196, 2016

4.Miwa, H.,Era, T. Mesoderm differentiation from hiPS Cells. Methods in Molecular Biology 1357:403-413, 2016

5.Maeda, Y., Motoyama, K., Higashi, T., Horikoshi, Y., Takeo, T., Nakagata, N., Kurauchi, Y., Katsuki, H., Kondo, Y., Ishitsuka, Y., Irie, T., Era, T., Arima, H. Feasibility study of cyclodextrins as active pharmaceutical ingredients for the treatment of GM1-gangliosidosis. Asian Journal of Pharmaceutical Sciences 11(1):183-184, 2016

6.Hamauchi, S., Shichinohe, H., Uchino, H., Yamaguchi, S., Nakayama, N., Kazumata, K., Osanai, T., Abumiya, T., Houkin, K., Era, T. Cellular functions and gene and protein expression profiles in endothelial cells derived from Moyamoya disease-specific iPS cells. PLoS ONE 11(9), 2016

 

発生医学研究所 細胞医学分野 中尾 光善
1.Satou, Y., Miyazato, P., Ishihara, K., Yaguchi, H., Melamed, A., Miura, M., Fukuda, A., Nosaka, K., Watanabe, T., Rowan, A.G., Nakao, M., Bangham, C.R.M. The retrovirus HTLV-1 inserts an ectopic CTCF-binding site into the human genome. Proceedings of the National Academy of Sciences of the United States of America 113(11): 3054-3059, 2016

2.Kosumi, K., Baba, Y., Sakamoto, A., Ishimoto, T., Harada, K., Nakamura, K., Kurashige, J., Hiyoshi, Y., Iwatsuki, M., Iwagami, S., Sakamoto, Y., Miyamoto, Y., Yoshida, N., Oki, E., Watanabe, M., Hino, S., Nakao, M., Baba, H. Lysine-specific demethylase-1 contributes to malignant behavior by regulation of invasive activity and metabolic shift in esophageal cancer. International Journal of Cancer 138(2):428-439 ,2016

3.Xi, Y., Shen, W., Ma, L., Zhao, M., Zheng, J., Bu, S., Hino, S., Nakao, M. HMGA2 promotes adipogenesis by activating C/EBPβ-mediated expression of PPARγ. Biochemical and Biophysical Research Communications 472(4):617-623, 2016

4.Hino, S., Kohrogi, K.,Nakao, M. Histone demethylase LSD1 controls the phenotypic plasticity of cancer cells. Cancer Science 107(9):1187-1192, 2016

5.Miyazato, P., Katsuya, H., Fukuda, A., Uchiyama, Y., Matsuo, M., Tokunaga, M., Hino, S., Nakao, M., Satou, Y. Application of targeted enrichment to next-generation sequencing of retroviruses integrated into the host human genome. Scientific Reports 6, 2016

6.Nakayama, T., Saitoh, N., Morotomi-Yano, K., Yano, K.-I., Nakao, M., Saitoh, H. Nuclear extrusion precedes discharge of genomic DNA fibers during tunicamycin-induced neutrophil extracellular trap-osis (NETosis)-like cell death in cultured human leukemia cells. Cell Biology International 40(5):597-602, 2016

7.Nakamura, K., Baba, Y., Kosumi, K., Harada, K., Shigaki, H., Miyake, K., Kiyozumi, Y., Ohuchi, M., Kurashige, J., Ishimoto, T., Iwatsuki, M., Sakamoto, Y., Yoshida, N., Watanabe, M., Nakao, M., Baba, H. UHRF1 regulates global DNA hypomethylation and is associated with poor prognosis in esophageal squamous cell carcinoma. Oncotarget 7(36):57821-57831, 2016

 

視機能病態学分野 谷原 秀信
1.Tanihara, H., Inoue, T., Yamamoto, T., Kuwayama, Y., Abe, H., Fukushima, A., Suganami, H., Araie, M. One-year clinical evaluation of 0.4% ripasudil (K-115) in patients with open-angle glaucoma and ocular hypertension. Acta Ophthalmologica 94(1): e26-e34, 2016.

2.Kaneko, Y., Ohta, M., Inoue, T., Mizuno, K., Isobe, T., Tanabe, S., Tanihara, H. Effects of K-115 (Ripasudil), a novel ROCK inhibitor, on trabecular meshwork and Schlemms canal endothelial cells. Scientific Reports 6, 2016

3.Ohira, S., Inoue, T., Iwao, K., Takahashi, E., Tanihara, H. Factors influencing aqueous proinflammatory cytokines and growth factors in uveitic glaucoma. PLoS ONE 11(1), 2016

4.Iraha, S., Hirami, Y., Ota, S., Sunagawa, G.A., Mandai, M., Tanihara, H., Takahashi, M., Kurimot, Y. Efficacy of valproic acid for retinitis pigmentosa patients: A pilot study. Clinical Ophthalmology 10(-):1375-1384, 2016

5.Kitamura, F., Inoue, T., Kuroda, U., Tanihara, H. Angle closure caused by a plateau-like iris associated with an enlarged Soemmering’s ring: A case report. BMC Ophthalmology 16(1), 2016

6.Nishisako, M., Meguro, A., Nomura, E., Yamane, T., Takeuchi, M., Ota, M., Kashiwagi, K., Mabuchi, F., Iijima, H., Kawase, K., Yamamoto, T., Nakamura, M., Negi, A., Sagara, T., Nishida, T., Inatani, M., Tanihara, H., Aihara, M., Araie, M., Fukuchi, T., Abe, H., Higashide, T., Sugiyama, K., Kanamoto, T., Kiuchi, Y., Iwase, A., Chin, S., Ohno, S., Inoko, H., Mizuki, N. SLC1A1 Gene Variants and Normal Tension Glaucoma: An Association Study. Ophthalmic Genetics 37(2):194-200, 2016

7.Matsumura, R., Inoue, T., Tanihara, H. Bleb-like finding caused by idiopathic scleral thinning in an eye with absolute glaucoma. Journal of Glaucoma 25(3):e288-e290, 2016

8.Fujimoto, T., Inoue, T., Inoue-Mochita, M., Tanihara, H. Live cell imaging of actin dynamics in dexamethasone-treated porcine trabecular meshwork cells. Experimental Eye Research 145(-):393-400, 2016

9.Kojima, S., Inoue, T., Kikuta, J., Furuya, M., Koga, A., Fujimoto, T., Ueta, M., Kinoshita, S., Ishii, M., Tanihara, H. Visualization of intravital immune cell dynamics after conjunctival surgery using multiphoton microscopy. Investigative Ophthalmology and Visual Science 57(3):1207-1212, 2016

10.Kawaji, T., Sato, T., Tanihara, H. Sutureless intrascleral intraocular lens fixation with lamellar dissection of scleral tunnel. Clinical Ophthalmology 10:227-231, 2016

11.Takahashi, E., Fukushima, A., Haga, A., Inomata, Y., Ito, Y., Fukushima, M., Tanihara, H. Effects of mechanical stress and vitreous samples in retinal pigment epithelial cells. Biochemical and Biophysical Research Communications 470(3):569-574, 2016

12.Nishizawa, A., Inoue, T., Ohira, S., Takahashi, E., Saruwatari, J., Iwao, K., Tanihara, H. The influence of phacoemulsification on surgical outcomes of trabeculectomy with mitomycin-C for uveitic glaucoma. PLoS ONE 11(3):-, 2016

13.Kashiwagi, K., Kogure, S., Mabuchi, F., Chiba, T., Yamamoto, T., Kuwayama, Y., Araie, M., Aihara, M., Abe, H., Iwase, A., Uji, Y., Katsushima, H., Kitazawa, Y., Sawaguchi, S., Shimizu, H., Shirato, S., Sugiyama, K., Suzuki, Y., Tanihara, H., Tawara, A., Tomita, G., Tomidokoro, A., Negi, A., Fukuchi, T., Matsumoto, C., Mishima, H.K., Yoshikawa, K., Yoshitomi, T., Inoue, Y., Aoyama, Y., Kawase, K., Ban, T., Sawada, A., Mochizuki, K., Futa, R., Nakamoto, K., Sugiyama, T., Kojima, S., Hirotsuji, N., Kano, K., Ohura, A., Kawai, E., Nakata, A., Kuwamura, R., Okuno, K., Yawata, K., Matsumoto, S., Abe, H., Fukuchi, T., Shirakashi, M., Ueda, J., Tanaka, T., Negi, A., Nakamura, M., Kanamori, A., Yamada, Y., Tawara, A., Kubota, T., Tou, N., Kimura, T., Furusawa, C., Inoue, N., Kawabata, K., Tachibana, N., Matsuda, A., Seto, T., Kimura, S., Ueno, S., Tokuda, N., Inoue, J., Sugiyama, K., Ohkubo, S., Higashide, T., Saito, Y., Takeda, H., Nagata, A., Uji, Y., Tsukitome, H., Ito, K., Ikesugi, K., Matsumura, M., Nambu, H., Hamanaka, T., Omata, T., Watanabe, Y., Sakuma, K., Amari, Y., Takahashi, G., Noro, T., Miyata, H., Hirasawa, M., Ohtake, Y., Yuki, K., Tomita, G., Aihara, M., Tomidokoro, A., Matsuo, H., Atarashi, T., Mayama, C., Yamagami, J., Okinami, S., Ishikawa, S., Shoji, N., Morita, T., Otsuki, T., Suzuki, H., Naruse, R., Yamakawa, Y., Kasahara, M., Yoshitomi, T., Suzuki, Y., Shimizu, S., Mishima, H.K., Kiuchi, Y., Mochizuki, H., Sone, T., Tsukamoto, H., Okada, K., Sakai, H., Arakaki, Y., Ishikawa, S., Tanihara, H., Inatani, M., Inoue, T., Koga, T., Kawase, K., Aoyama, A., Yamazaki, Y., Okajima, Y., Mizuki, K., Hayamizu, F., Nakagami, T., Mori, K., Ikeda, Y., Ueno, M., Imai, K., Kawai, K., Sueno, T., Saito, N., Otori, Y. Change in visual acuity and associated risk factors after trabeculectomy with adjunctive mitomycin C. Acta Ophthalmologica 94(7):e561-e570, 2016

14.Haga, A., Takahashi, E., Inomata, Y., Kawahara, K., Tanihara, H. Differentiated expression patterns and phagocytic activities of type 1 and 2 microglia. Investigative Ophthalmology and Visual Science 57(6):2814-2823, 2016
15.Futakuchi, A., Inoue, T., Fujimoto, T., Inoue-Mochita, M., Kawai, M., Tanihara, H. The effects of ripasudil (K-115), a Rho kinase inhibitor, on activation of human conjunctival fibroblasts. Experimental Eye Research 149:107-115, 2016

16.Tanihara, H., Abe, I. The Origin of the Disorder Named “”Ryoku-naisho (Glaucoma)””. Nippon Ganka Gakkai zasshi 120(5):369-381, 2016

17.Tsutsumi, T., Iwao, K., Hayashi, H., Kirihara, T., Kawaji, T., Inoue, T., Hino, S., Nakao, M., Tanihara, H. Potential neuroprotective effects of an LSD1 inhibitor in retinal ganglion cells via p38 MAPK activity. Investigative Ophthalmology and Visual Science 57(14):6461-6473, 2016

18.Matsumoto, M., Inoue, T., Kojima, S., Nakashima, K.-I., Fukushima, A., Tanihara, H. Prospective observation of filtration blebs in high-risk eyes with limbal-based conjunctival flap. Canadian Journal of Ophthalmology 51(6):431-437, 2016

19.Fujimoto, T., Inoue, T., Maki, K., Inoue-Mochita, M., Tanihara, H. Vascular endothelial growth factor – A increases the aqueous humor outflow facility. PLoS ONE 11(9), 2016

 

遺伝子機能応用学分野  甲斐 広文
1.Suzuki, S., Sargent, R.G., Illek, B., Fischer, H., Esmaeili-Shandiz, A., Yezzi, M.J., Lee, A., Yang, Y., Kim, S., Renz, P., Qi, Z., Yu, J., Muench, M.O., Beyer, A.I., Guimarães, A.O., Ye, L., Chang, J., Fine, E.J., Cradick, T.J., Bao, G., Rahdar, M., Porteus, M.H., Shuto, T., Kai, H., Kan, Y.W., Gruenert, D.C. TALENs facilitate single-step seamless SDF correction of F508del CFTR in airway epithelial submucosal gland cell-derived CF-iPSCs. Molecular Therapy – Nucleic Acids 5, 2016.

2.Fukuda, R., Suico, M.A., Kai, Y., Omachi, K., Motomura, K., Koga, T., Komohara, Y., Koyama, K., Yokota, T., Taura, M., Shuto, T., Kai, H. Podocyte p53 Limits the Severity of Experimental Alport Syndrome. Journal of the American Society of Nephrology 27(1):144-157, 2016

3.Suico, M.A., Taura, M., Kudo, E., Gotoh, K., Shuto, T., Okada, S., Kai, H. The ETS factor myeloid Elf-1-Like Factor (MEF)/Elf4 is transcriptionally and functionally activated by hypoxia. Biological and Pharmaceutical Bulletin 39(4):641-647, 2016

4.Kondo, T., Goto, R., Ono, K., Kitano, S., Suico, M.A., Sato, M., Igata, M., Kawashima, J., Motoshima, H., Matsumura, T., Kai, H., Araki, E. Activation of heat shock response to treat obese subjects with type 2 diabetes: A prospective, frequency-escalating, randomized, open-label, triple-arm trial. Scientific Reports 6, 2016

5.Shuto, T., Kamei, S., Nohara, H., Fujikawa, H., Tasaki, Y., Sugahara, T., Ono, T., Matsumoto, C., Sakaguchi, Y., Maruta, K., Nakashima, R., Kawakami, T., Suico, M.A., Kondo, Y., Ishigami, A., Takeo, T., Tanaka, K.-I., Watanabe, H., Nakagata, N., Uchimura, K., Kitamura, K., Li, J.-D., Kai, H. Pharmacological and genetic reappraisals of protease and oxidative stress pathways in a mouse model of obstructive lung diseases. Scientific Reports 6, 2016

6.Tasaki Y, Wang WY, Konduru AS, Komatsu K, Matsuyama S, Kai H, Li JD cAMP-dependent protein kinase A acts as a negative regulator for nontypeable Haemophilus influenzae-induced GM-CSF expression via inhibition of MEK-ERK signaling pathway. Integr Mol Med. 2016.DOI: 10.15761/IMM.1000261

 

微生物薬学分野  大槻 純男
1.Uchida, Y., Toyohara, T., Ohtsuki, S., Moriyama, Y., Abe, T., Terasaki, T. Quantitative Targeted Absolute Proteomics for 28 Transporters in Brush-Border and Basolateral Membrane Fractions of Rat Kidney. Journal of Pharmaceutical Sciences 105(2): 1011-1016, 2016

2.Nakamura, K., Hirayama-Kurogi, M., Ito, S., Kuno, T., Yoneyama, T., Obuchi, W., Terasaki, T., Ohtsuki, S. Large-scale multiplex absolute protein quantification of drug-metabolizing enzymes and transporters in human intestine, liver, and kidney microsomes by SWATH-MS: Comparison with MRM/SRM and HR-MRM/PRM. Proteomics 16(15-16):2106-2117, 2016

3.Akazawa, T., Uchida, Y., Tachikawa, M.,Ohtsuki, S., Terasaki, T. Quantitative targeted absolute proteomics of transporters and pharmacoproteomics-based reconstruction of P-glycoprotein function in mouse small intestine. Molecular Pharmaceutics 13(7):2443-2456, 2016

4.Yoneyama, T., Ohtsuki, S., Honda, K., Kobayashi, M., Iwasaki, M., Uchida, Y., Okusaka, T., Nakamori, S., Shimahara, M., Ueno, T., Tsuchida, A., Sata, N., Ioka, T., Yasunami, Y., Kosuge, T., Kaneda, T., Kato, T., Yagihara, K., Fujita, S., Huang, W., Yamada, T., Tachikawa, M., Terasaki, T. Identification of IGFBP2 and IGFBP3 as compensatory biomarkers for CA19-9 in early-stage pancreatic cancer using a combination of antibody-based and LC-MS/MS-based proteomics. PLoS ONE,11(8):e0161009, 2016

5.Kuno, T., Hirayama-Kurogi, M., Ito, S., Ohtsuki, S. Effect of Intestinal Flora on Protein Expression of Drug-Metabolizing Enzymes and Transporters in the Liver and Kidney of Germ-Free and Antibiotics-Treated Mice. Molecular Pharmaceutics 13(8):2691-2701, 2016

6.Kuroda, H., Tachikawa, M., Uchida, Y., Inoue, K., Ohtsuka, H., Ohtsuki, S., Unno, M., Terasaki, T. All-trans retinoic acid enhances gemcitabine cytotoxicity in human pancreatic cancer cell line AsPC-1 by up-regulating protein expression of deoxycytidine kinase. European Journal of Pharmaceutical Sciences, 103:116-121, 2017

 

神経分科学分野  太田 訓正
1.Acharjee, U.K., Felemban, A.A., Riyadh, A.M., Ohta, K. Regulation of the neural niche by the soluble molecule Akhirin. Development Growth and Differentiation 58(5):463-468, 2016

 

多能性幹細胞分野 丹羽 仁史
1.Nakai-Futatsugi, Y., Niwa, H. Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells. Stem Cell Reports 6(4): 483-495, 2016

2.Niwa, H., Nakamura, A., Urata, M., Shirae-Kurabayashi, M., Kuraku, S., Russell, S., Ohtsuka, S. The evolutionally-conserved function of group B1 Sox family members confers the unique role of Sox2 in mouse ES cells. BMC Evolutionary Biology 16(1), 2016

3.Sekinaka, T., Hayashi, Y., Noce, T., Niwa, H., Matsui, Y. Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment. Scientific Reports 6, 2016