国際先端研究拠点_論文2015年度 - 熊本大学発生医学研究所

国際先端研究拠点

国立大学法人熊本大学　国際先端研究拠点
“幹細胞を用いた臓器再建と次世代医療・創薬を目指す国際的研究教育拠点”
“International Research Core for Stem Cell-based Developmental Medicine”

国際先端研究拠点ホームへ

平成27年度(2015年度)

発生医学研究所　腎臓発生分野　西中村隆一

Akiyama R, Kawakami H, Wong J, Oishi I, Nishinakamura R, Kawakami Y. Sall4-Gli3 system in early limb progenitors is essential for the development of limb skeletal elements. Proc. Natl. Acad. Sci. USA. 112(16):5075-5080, 2015.
Hayata T, Ezura Y, Asashima M, Nishinakamura R, Noda M. Dullard/Ctdnep1 regulates endochondral ossification via suppression of TGF-β signaling. J. Bone. Miner. Res. 30(5):947, 2015.
Escobar D, Hepp MI, Farkas C, Campos T, Sodir NM, Morales M, Álvarez CI, Swigart L, Evan GI, Gutiérrez JL, Nishinakamura R, Castro AF, Pincheira R. Sall2 is required for proapoptotic Noxa expression and genotoxic stress-induced apoptosis by doxorubicin. Cell Death Dis. 6:e1816, 2015.
Sweetwyne MT, Gruenwald A, Niranjan T, Nishinakamura R, Strobl LJ, Susztak K. Notch1 and Notch2 in podocytes play differential roles during diabetic nephropathy development. Diabetes. Epub ahead of print
Hirsch S, El-Achkar TM, Robbins L, Basta J, Heitmeier MR, Nishinakamura R, Rauchman M. A mouse model of Townes-Brocks syndrome expressing a truncated mutant Sall1 protein is protected from acute kidney injury. Am. J. Physiol. Renal Physiol. Epub ahead of print
Tanigawa S, Sharma N, Hall MD, Nishinakamura R, and Perantoni AO. Preferential propagation of competent SIX2+ nephronic progenitors by LIF/ROCKi treatment of the metanephric mesenchyme. Stem Cell Reports 5(3):435-447, 2015.
Sharmin S, Taguchi A, Kaku Y, Yoshimura Y, Ohmori T, Sakuma T, Mukoyama M, Yamamoto T, Kurihara H, and Nishinakamura R. Human induced pluripotent stem cell-derived podocytes mature into vascularized glomeruli upon experimental transplantation. J. Am. Soc. Nephrol. Epub ahead of print.
Ohmori T, Tanigawa S, Kaku Y, Fujimura S, and Nishinakamura R. Sall1 in renal stromal progenitors non-cell autonomously restricts the excessive expansion of nephron progenitors. Sci. Rep. 5: 15676, 2015. doi:10.1038/srep15676

発生医学研究所　幹細胞誘導分野　江良択実
1. Sato Y, Kobayashi H, Higuchi T, Shimada Y, Era T, Kimura S, Eto Y, Ida H, Ohashi T. Disease modeling and lentiviral gene transfer in patient-specific induced pluripotent stem cells from late-onset Pompe disease patient. Mol. Ther. Methods Clin. Dev. 2:15023, 2015.
2. Yonehara A, Tanaka Y, Kulkeaw K, Era T, Nakanishi Y, Sugiyama D. Aloe vera Extract Suppresses Proliferation of Neuroblastoma Cells In Vitro. Anticancer Res. 8:4479-4485, 2015
3. Miwa H, Era T. Generation and characterization of PDGFRα-GFPCreERT2 knock-In mouse line. Genesis 53:329-36, 2015.

Maeda Y, Motoyama K, Higashi T, Horikoshi Y, Takeo T, Nakagata N, Kurauchi Y, Katsuki H, Ishitsuka Y, Kondo Y, Irie T, Furuya H, Era T, Arima H. Effects of cyclodextrins on GM1-gangliosides in fibroblasts from GM1-gangliosidosis patients.J.Pharm. Pharmacol. 67:1133-1142, 2015.

Soga M, Ishitsuka Y, Hamasaki M, Yoneda K, Furuya H, Matsuo M, Ihn H, Fusaki N, Nakamura K, Nakagata N, Endo F, Irie T, Era T. HPGCD outperforms HPBCD as a potential treatment for Niemann-Pick disease type C during disease modeling with iPS cells. Stem Cells 33: 1075-1088, 2015.

発生医学研究所　細胞医学分野　中尾光善
1. Matsumura Y, Nakaki R, Inagaki T, Yoshida A, Kano Y, Kimura H, Tanaka T, Tsutsumi S, Nakao M, Doi T, Fukami K, Osborne T.F, Kodama T, Aburatani H, Sakai J. H3K4/H3K9me3 bivalent chromatin domains targeted by lineage-specific DNA methylation pauses adipocyte differentiation. Mol. Cell. (in press).

2 Koga T, Suico M.A, Shimasaki S, Watanabe E, Kai Y, Koyama K, Omachi K, Morino-Koga S, Sato T, Shuto T, Mori K, Hino S, Nakao M, Kai H. ER stress induces SIRT1 expression via PI3K-Akt-GSK3 signaling pathway and promotes hepatocellular injury. J. Biol. Chem. (in press).
3. Ali T.F, Iwamaru K, Ciftci H.I, Koga R, Matsumoto M, Oba Y, Kurosaki H, Fujita M, Okamoto Y, Umezawa K, Nakao M, Hide T, Makino K, Kuratsu J, Abdel-Aziz M, Abuo-Rahma Gel-D, Beshr E.A, Otsuka M. Novel metal chelating molecules with anticancer activity. Striking effect of the imidazole substitution of the histidine-pyridine-histidine system. Bioorg. Med. Chem. 23: 5476-5482, 2015.
4. Kosumi K, Baba Y, Ishimoto T, Sakamoto A, Harada K, Kurashige J, Hiyoshi Y, Iwatsuki M, Iwagami S, Miyamoto Y, Sakamoto Y, Yoshida N, Watanabe M, Hino S, Nakao M, Baba H. Lysine-specific demethylase-1 contributes to malignant behavior by regulation of invasive activity and metabolic shift in esophageal cancer. Int. J. Cancer. (in press).
5. Kitamura H, Matsumori H, Kalendova A, Hozak P, Goldberg I.G, Nakao M, Saitoh N, Harata M. The actin family protein ARP6 contributes to the structure and the function of the nucleolus. Biochem. Biophys. Res. Commun. 464: 554-560, 2015.

6. Wagatsuma K, Tani-ichi S, Liang B, Shitara S, Ishihara K, Abe M, Miyachi H, Kitano S, Hara T, Nanno M, Ishikawa H, Sakimura K, Nakao M, Kimura H, Ikuta K. STAT5 orchestrates local epigenetic changes for chromatin accessibility and rearrangements by direct binding to the TCR locus. J. Immunol. 195: 1804-1814, 2015.

7. Murata A, Baba Y, Ishimoto T, Miyake K, Kosumi K, Harada K, Kurashige J, Iwagami S, Sakamoto Y, Miyamoto Y, Yoshida N, Yamamoto M, Oda S, Watanabe M, Nakao M, Baba H. TET family proteins and 5-hydroxymethylcytosine in esophageal squamous cell carcinoma. Oncotarget. 6: 23372-23382, 2015.

8. Takebayashi S, Tanaka H, Hino S, Nakatsu Y, Igata T, Sakamoto A, Narita M, Nakao M. Retinoblastoma protein promotes oxidative phosphorylation through up-regulation of glycolytic genes in oncogene-induced senescent cells. Aging Cell. 14: 689-697, 2015.

9. Tomita S, Abdalla M.O, Fujiwara S, Matsumori H, Maehara K, Ohkawa Y, Iwase H, Saitoh N, Nakao M. A cluster of non-coding RNAs activates the ESR1 locus during breast cancer adaptation to hormone deprivation. Nat. Commun. 6: 6966, 2015.

10. Sakamoto A, Hino S, Nagaoka K, Anan K, Takase R, Matsumori H, Ojima H, Kanai Y, Arita K, Nakao M. Lysine demethylase LSD1 coordinates glycolytic and mitochondrial metabolism in hepatocellular carcinoma cells. Cancer Res. 75: 1445-1456, 2015.

11. Nagaoka K, Hino S, Sakamoto A, Anan K, Takase R, Umehara T, Yokoyama S, Sasaki Y, Nakao M. Lysine-specific demethylase LSD2 suppresses lipid influx and metabolism in hepatic cells. Mol. Cell. Biol. 35: 1068-1080, 2015.

視機能病態学分野　谷原秀信

Kojima S, Inoue T, Nakashima K, Fukushima A, Tanihara H. Filtering blebs using 3-dimensional anterior-segment optical coherence tomography: a prospective investigation. JAMA Ophthalmology 133: 148-156, 2015.
Tanihara H, Inoue T, Yamamoto T, Kuwayama Y, Abe H, Suganami H, Araie M for the K-115 Clinical Study Group. Intra-ocular pressure-lowering effects of a Rho kinase inhibitor, ripasudil (K-115), over 24 hours in primary open-angle glaucoma and ocular hypertension: a randomized, open-label, crossover study. Acta Ophthalmologica 93: e254-269, 2015.
Tasaki M, Ueda M, Matsumoto K, Kawaji T, Misumi Y, Eiki D, Suenaga G, Obayashi K, Yamashita T, Tanihara H, Ando Y. Clinico-histopathological and biochemical analyses of corneal amyloidosis in gelatinous drop-like corneal dystrophy. Amyloid 22: 67-69, 2015.
Nakashima K, Inoue T, Fukushima A, Hirakawa S, Kojima S, Tanihara H. Evaluation of filtering blebs exhibiting transconjunctival oozing using anterior segment optical coherence tomography. Graefe’s Archive of Clinical and Experimental Ophthalmology 253: 439-445, 2015.
Inoue T, Tanihara H, Tokushige H, Araie M. Efficacy and safety of SNJ-1656 in primary open-angle glaucoma or ocular hypertension. Acta Ophthalmologica 93:e393-395, 2015.
Tanihara H, Inoue T, Yamamoto T, Kuwayama Y, Abe H, Suganami H, Araie M for the K-115 Clinical Study Group. Additive intraocular pressure-lowering effects of the Rho kinase inhibitor, ripasudil (K-115), combined with timolol or latanoprost: a report of 2 randomized clinical trials. JAMA Ophthalmology 133:755-61, 2015.
Inoue-Mochita M, Inoue T, Fujimoto T, Kameda T, Awai-Kasaoka N, Ohtsu N, Kimoto K, Tanihara H. p38 MAP kinase inhibitor suppresses transforming growth factor-β2-induced type 1 collagen production in trabecular meshwork cells. PLoS One 10: e0120774, 2015
Takahashi E, Haga A, Tanihara H. Merlin regulates epithelial-to-mesenchymal transition of ARPE-19 cells via TAK1-p38MAPK-mediated activation. Investigative Ophthalmology and Visual Science 56: 2449-2458, 2015.
Ohira S, Inoue T, Shobayashi K, Iwao K, Fukushima M, Tanihara H. Simultaneous increase in multiple proinflammatory cytokines in the aqueous humor in neovascular glaucoma with and without intravitreal bevacizumab injection. Investigative Ophthalmology and Visual Science 56: 3541-3548. 2015.
Cho HK, Kojima S, Inoue T, Fukushima A, Kee C, Tanihara H. Effect of laser suture lysis on filtration openings: a prospective three-dimensional anterior segment optical coherence tomography study. Eye 29:1220-1225, 2015.
Shobayashi K, Inoue T, Kawai M, Iwao K, Ohira S, Kojima S, Kuroda U, Nakashima　K, Tanihara H. Postoperative Changes in Aqueous Monocyte Chemotactic Protein-1　Levels and Bleb Morphology after Trabeculectomy vs. Ex-PRESS Shunt Surgery. PLoS One　10:e0139751, 2015.
Tanihara H, Inoue T, Yamamoto T, Kuwayama Y, Abe H, Fukushima A, Suganami H,　Araie M; K-115 Clinical Study Group. One-year clinical evaluation of 0.4% ripasudil (K-115) in patients with open-angle glaucoma and ocular hypertension. Acta Ophthalmol. (in press)
Takihara Y, Inatani M, Eto K, Inoue T, Kreymerman A, Miyake S, Ueno S, Nagaya M, Nakanishi A, Iwao K, Takamura Y, Sakamoto H, Satoh K, Kondo M, Sakamoto T,　Goldberg JL, Nabekura J, Tanihara H. In vivo imaging of axonal transport of　mitochondria in the diseased and aged mammalian CNS. Proc. Natl. Acad. Sci. U S A. 112:10515-10520, 2015.
Fujimoto T, Inoue T, Kameda T, Kasaoka N, Inoue-Mochita M, Tsuboi N, Tanihara H. Erratum. Involvement of RhoA/Rho-Associated Kinase Signal Transduction Pathway in Dexamethasone-Induced Alterations in Aqueous Outflow. Investigative Ophthalmology and Visual Science 56:1593, 2015.

遺伝子機能応用学分野　甲斐広文

Kadowaki H, Nagai A, Maruyama T, Takami Y, Fitrah PS, Kato H, Honda A, Hatta T, Natsume T, Sato T, Kai H, Ichijo H, Nishitoh H. Preemptive quality control protects the ER from protein overload via the proximity of ERAD components and SRP. Cell Reports (in press), 2015.
Fukuda R, Suico MA, Kai Y, Omachi K, Motomura K, Koga T, Komohara Y, Koyama K, Yokota T, Taura M, Shuto T, Kai H. Podocyte p53 Limits the Severity of Experimental Alport Syndrome. J. Am. Soc. Nephrol. (in press), 2015.
Miyata SS, Miyata M, Lee BC, Xu H, Kai H, Yan C, Li JD. Cross-talk between PKA-Cβ and p65 mediates synergistic induction of PDE4B by roflumilast and NTHi. Proc. Natl. Acad. Sci. U S A. 112(14):E1800-9, 2015..
Koga T, Suico MA, Shimasaki S, Watanabe E, Kai Y, Koyama K, Omachi K, Morino-Koga S, Sato T, Shuto T, Mori K, Hino S, Nakao M, Kai H. ER stress induces SIRT1 expression via PI3K-Akt-GSK3b signaling pathway and promotes hepatocellular injury. J. Biol. Chem. pii: jbc.M115.664169, 2015.
Yokoyama T, Shuto T, Kai H, et al. Structural stabilization of transthyretin by a new compound, 6-benzoyl-2-hydroxy-1H-benzo[de]isoquinoline-1,3(2H)-dione. J. Pharmacol. Sci. (in press), 2015.
Suzuki S, Shuto T, Kai H, et al. TALENs facilitate single-step seamless SDF correction of F508del CFTR in airway epithelial submucosal gland cell-derived CF-iPSCs. Molecular Therapy Nucleic Acids. (in press), 2015.

微生物薬学分野　大槻　純男

Ohmine K, Kawaguchi K, Ohtsuki S, Motoi F, Ohtsuka H, Kamiie J, Abe T, Unno M, and Terasaki T. Quantitative Targeted Proteomics of Pancreatic Cancer: Deoxycytidine Kinase Protein Level Correlates to Progression-Free Survival of Patients Receiving Gemcitabine Treatment. Mol. Pharm. 12:3282-3291, 2015.
Oyama T, Negishi E, Onigahara H, Kusano N, Miyoshi Y, Mita M, Nakazono M, Ohtsuki S, Ojida A, Lindner W, and Hamase K. Design and synthesis of a novel pre-column derivatization reagent with a 6-methoxy-4-quinolone moiety for fluorescence and tandem mass spectrometric detection and its application to chiral amino acid analysis. J. Pharm. Biomed. Anal. 116:71-79 , 2015.
Uchida Y, Ito K, Ohtsuki S, Kubo Y, Suzuki T, and Terasaki T. Major involvement of Na(+) -dependent multivitamin transporter (SLC5A6/SMVT) in uptake of biotin and pantothenic acid by human brain capillary endothelial cells. J. Neurochem. 134:97-112, 2015.
Uchida Y, Ohtsuki S, Kamiie J, Ohmine K, Iwase R, and Terasaki T. Quantitative targeted absolute proteomics for 28 human transporters in plasma membrane of Caco-2 cell monolayer cultured for 2, 3, and 4 weeks. Drug Metab. Pharmacokinet. 30:205-208, 2015.
Kubo Y, Ohtsuki S, Uchida Y, and Terasaki T. Quantitative Determination of Luminal and Abluminal Membrane Distributions of Transporters in Porcine Brain Capillaries by Plasma Membrane Fractionation and Quantitative Targeted Proteomics. J. Pharm. Sci. 104:3060-3068 , 2015.
Kaneko Y, Tachikawa M, Akaogi R, Fujimoto K, Ishibashi M, Uchida Y, Couraud PO, Ohtsuki S, Hosoya K, and Terasaki T. Contribution of pannexin 1 and connexin 43 hemichannels to extracellular calcium-dependent transport dynamics in human blood-brain barrier endothelial cells. J. Pharmacol. Exp. Ther. 353:192-200, 2015.

神経分科学分野　太田　訓正

Shinmyo Y#, Riyadh A#, Giasuddin A, Iftekhar Bin N, Mahmud H, Takebayashi H, Kawasaki H, Ohta K, Tanaka H. Draxin from neocortical neurons controls the guidance of thalamocortical projections into the neocortex. Nat. Commun. (in press), 2015.
Acharjee U K, Gejima R, Abdulhaleem M F A, Riyadh M A, Tanaka H, Ohta K. Tsukushi expression is dependent on Notch signaling and oscillated in the presomitic mesoderm during chick somitogenesis. Biochem. Biophys. Res. Commun. 465: 625-630, 2015.
Ito N, Ohta K. Reprogramming of human somatic cells by bacteria. Develop. Growth Differ. 57: 305-312, 2015.
Abdulhaleem M F A, Song X, Kawano R, Uezono N, Ito A, Ahmed G, Hossain M, Nakashima K, Tanaka H, Ohta K. Akhirin regulates the proliferation and differentiation of neural stem cells in intact and injured mouse spinal cord. Dev. Neurobiol. 75: 494-504, 2015.

多能性幹細胞分野　丹羽仁史

Kinoshita M, Shimosato D, Yamane M, Niwa H. Sox7 is dispensable for primitive endoderm differentiation from mouse ES cells. BMC Dev. Biol. 15: 37, 2015.
Ohtsuka S, Niwa H. The differential activation of intracellular signaling pathways confers the permissiveness of embryonic stem cell derivation from different mouse strains. Development 142: 431-437, 2015.