日 時: 平成29年7月12日(水) 16:00～17:00
場 所: 発生医学研究所１階 カンファレンスルーム
The degree and dynamics of translational control during mammalian development remain poorly understood. There is a lack of understanding for how translational control contributes to the cell fate decisions and complex body plan formation. Here, I will show our work, which monitored translation of the mammalian genome during cell specification and organization into tissues in vivo. This identified unexpected and pervasive translational regulation of most of the core signaling circuitry including Shh, Wnt, Hippo, PI3K and MAPK pathways, revealing a new layer of control signaling networks. We further identify and functionally characterize a complex landscape of upstream open reading frames (uORFs) across 5’-untranslated regions (UTRs) of key signaling components. Focusing on the Shh pathway, we demonstrate the importance of uORFs within the major SHH receptor, Ptch1, in control of cell signaling and neuronal differentiation. Finally, we show that translation diversifies special gene expression at the same stage of embryonic development. Additionally I will introduce the new work to change the current dogma of translation, in which ribosomes homogeneously contain 4 rRNAs and 80 ribosomal proteins. We will show the heterogeneity of the ribosome composition and preferential translation for the specific mRNAs. Together I will discuss the complexity and significance of translational regulation in the development.
Reference:
Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide (Mol Cell. Published on line 15 June 2017)
Pervasive translational regulation of the cell signalling circuitry underlies mammalian development (Nat Commun. 14, 14443, 2017)
RNA regulons in Hox 5′ UTRs confer ribosome specificity to gene regulation (Nature 517, 33-38, 2015)
連絡先 生殖発生分野 中村 輝 (内線6557)
共催：発生医学研究所 共同利用・共同研究拠点、熊本大学 国際先端研究拠点
