Naïve pluripotency is the emergent potential of individual cells to produce all lineages of the mature organism in a regulative manner. In rodents this initiation phase at the foundation of mammalian development can be captured and propagated in the form of embryonic stem (ES) cells. Shielded from differentiation cues by an appropriate culture environment, ES cells exhibit robust self-renewal, are substantially homogeneous, and are equipotent – a so-calledground state. Following release from ground state culture conditions ES cells enter a primedphase of pluripotency poised for multilineage differentiation and related to the gastrulating epiblast. I will describe efforts to generate human pluripotent stem cells with naïve features by resetting existing primed cell lines and by de novo derivation from human inner cell mass epiblast. I will also discuss our current studies into how mouse ES cells exit the naïve phase and transition towards lineage specification. Based on these observations I will propose a generic framework for pluripotency comprising three sequential phases: naïve, formative and primed.
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連絡先：発生医学研究所 多能性幹細胞分野 丹羽仁史（内線6620）
