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リエゾンラボ研究会
発表内容

Title:
Proliferation mechanism of mesenchymal progenitors

Takumi Era
Department of Cell Modulation, IMEG, Kumamoto University

Abstract:
Embryonic stem (ES) cells have the multiple potentials to give rise to a whole cell types in adult body and to undergo unlimited symmetrical divisions with pluri p otency. T he forced differentiation system of ES cell has been expected to use as a good tool to analyze the developmental mechanism into the specific cell lineage. However, as ES cell differentiation culture does not provide usefully positional information for cell type definition, this system definitely requires visible markers to identify and monitor the intermediates that present on the way of differentiation
We previously demonstrated that Platelet-derived growth factor receptor alpha (PDGFRα ) single positive fraction represented paraxial mesoderm during mouse embryogenesis. However, ES cell-derived PDGFRα + cells could not grow well under serum-containing condition. In this study, we have established a culture condition that allows PDGFRα+ cells to proliferate and differentiate into mesenchymal progenitors. In addition, t o investigate the molecular processes underlying mesoderm /mesenchymal development, we conducted the gene expression analyses in ES cell culture and succeeded in isolat ing a new molecule that is involved in mesenchymal differentiation. The null mutant of this molecule displayed neonatal lethality. Interestingly, the growth activity with the null fibroblast is shown to have an advantage over that with normal fibroblast. In this presentation, I will focus on mesenchymal development in ES cell culture and discuss the role of new molecule isolated from the culture in actual mesenchymal development.