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リエゾンラボ研究会
発表内容

Title:
Lysophosphatidic acid receptor-2 controls cell-cell adhesion stability via Lipoma prefered partnerduring Xenopus cranial neural crest cell migration

Sei Kuriyama Ph. D.
Associated professor
Department of Molecular Medicine and Biochemistry
Division of Oncoregulatory Medicine
Akita University Graduate School of Medicine and Faculty of Medicine

Abstract:
  Neural crest (NC) has undifferentiated, migratory and invasive characteristics, which shares many identities with typical cancer. Autotaxin ( ATX ), is a tumor cell motility factor isolated from melanoma, now known as lysophosphatidic acid (LPA)-producing enzyme, is expressed in Xenopus NC . This allows us speculate that ATX-LPA might work as a NC motility factor. Despite many suggestions of the roles of LPA in development, the knock-out mice of lpA 1/2 receptors don’t show criticalness in the embryogenesis. Here we show that lpA 2 /Edg-4 has a specific contribution on the cranial NC development via a novel binding partner, Lipoma prefered partner (LPP ). We found that antisense Edg-4 blocks the migration of NC by increasing the coherence of NC. LPP also has a role on the NC migration, and the association of LPP with s-catenin is Edg-4-dependent. LPP binds to the C-teminal of Edg-4 through the single phospho-serine of Edg-4 , which affects on the localization of LPP. Our findings suggest that LPA signaling in NC controls cell adhesion to switch premigratory into migratory NC. This might be a hidden role of lpA 2, which can be revealed in the lower vertebrate system.

Reference:
Kuriyama, S. & Mayor, R. Molecular analysis of neural crest migration. Philos Trans R Soc Lond B Biol Sci363, 1349-62 (2008).