筋発生再生分野 小野悠介准教授が昨年12月より発生研へ着任されました。下記の通り記念講演を開催します。
【Date】 Apr. 23 (Tue), 2019 16:30～17:00
【Venue】発生医学研究所 1Fカンファレンス室
【Title】 骨格筋幹細胞の機能的不均一性の理解に向けて
Toward understanding functional heterogeneity in muscle stem cells
【Speaker】 筋発生再生分野 小野 悠介 Yusuke Ono
【Abstract】
The resident muscle stem cells, called satellite cells, are located between the myofiber plasmalemma and the basal lamina, regulating muscle growth, hypertrophy, and regeneration by supplying new myonuclei. Accumulating evidence indicates that satellite cells exist as a genetically and functionally heterogeneous population among muscles, which is based not only on fiber-types, but also on embryonic origin. This heterogeneity may be linked to the body-region-specific pathological phenotypes in muscular dystrophy and age-related sarcopenia. We have been investigating the molecular mechanisms behind the region-specific regulation in adult muscles. Genome-wide gene expression analysis identified that the expression pattern of homeobox (Hox) genes in adult muscles almost recapitulates developmental identities. RNAi screening and genetically modified mouse models revealed that some Hox genes function in controlling cell-fate in satellite cells from somite-derived muscle, but not in those from paraxial head mesoderm-derived muscle, in adult mice. This region-specific regulation of satellite cells is conserved in human skeletal muscle. These findings indicate that Hox genes play a role in muscle regeneration by controlling satellite cell function in a body-region-specific manner. Satellite cells are also a heterogeneous population even within a single myofiber. Single-cell RNA-seq analysis further characterized transcriptional heterogeneity in the satellite cell population. The intra-population heterogeneity was more visible among satellite cells from aged mice compared with those from young mice. Our data therefore provide a molecular basis for satellite cell heterogeneity both among and within muscles. In this seminar, I will briefly describe recent findings and future directions of the laboratory.
【Contact】 発生医学研究所 多能性幹細胞 丹羽仁史 (Ext 6620)
