【Date】Jan. 13 (Fri), 2017 14:30～15:30
【Venue】Conference room, 1st floor, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University
【Abstract】
Pigs are considered as one of the best animals for generating models of human diseases and for providing organs for xenotransplantation. Currently, genetically modified pigs for biomedical applications have been mainly generated using the somatic cell nuclear transfer (SCNT) technique after the generation of engineered donor somatic cells. However, this approach requires complex micromanipulation techniques and a long time for preparing enough number of reconstructed embryos to be transferred to the surrogate pig. Recently, we have established a simple method for CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 gene editing in pigs that involves the introduction of Cas9 protein and single-guide RNA into in vitro fertilized zygotes by electroporation. The use of gene editing by electroporation of Cas9 protein (GEEP) resulted in highly efficient targeted gene disruption and was validated by the efficient production of Myostatin mutant pigs (Tanihara et al. 2016). We are now applying this method to generate pigs lacking specific organs including the kidney. These pigs are considered as potential hosts to generate human organs in vivo via complementing deficient organs with human pluripotent stem cells. In this seminar, we will introduce our recent studies and trials for generating genetically modified pigs by GEEP method.
References:
1.Tanihara F*, Takemoto T*, Kitagawa E, Rao S, Do LT, Onishi A, Yamashita Y, Kosugi C, Suzuki H, Sembon S, Suzuki S, Nakai M, Hashimoto M, Yasue A, Matsuhisa M, Noji S, Fujimura T, Fuchimoto D, and Otoi T. Somatic cell reprogramming-free generation of genetically modified pigs. (2016) Sci Adv 2:e1600803, *Equal contribution
【Contact】Dept. of Kidney Development, Ryuichi Nishinakamura (Ext. 6615)
このセミナーは「発生医学の共同研究拠点」の一環として行われます。
