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発表内容

Trans-omics: integration of multiple omic data on the basis of reaction kinetics

YUGI, Katsuyuki

1) Laboratory for Integrated Cellular Systems, RIKEN Center for Integrative Medical Sciences
2) Institute for Advanced Biosciences, Keio University
3) Department of Biological Sciences, University of Tokyo

 

Metabolic homeostasis is realized by global networks of molecular interactions across multiple ‘omic’ layers such as genome, transcriptome, proteome, and metabolome. We have proposed ‘trans-omics’, a methodology to reconstruct large-scale networks that span across the multiple omic layers on the basis of time-series omic data and reaction kinetics [1,2]. Here we show an application of trans-omics to reconstruction of a metabolic regulatory network that underlies acute insulin action in rat hepatoma FAO cells [3]. The network integrates phosphoprotome and metabolome data, and involves 13 protein kinases, 26 phosphorylated metabolic enzymes, and 35 allosteric effectors, resulting in quantitative changes in 44 metabolites. Mathematical modeling analysis predicted selective control of a subnetwork around phosphofructokinase by specific phosphorylation and allosteric regulation. Overall, we provide an unbiased method that reconstructs the trans-omic network from phosphoproteome and metabolome data, which will be applicable to other cellular responses.

 

Fig.1. A global landscape of the trans-omic network of acute insulin action in rat hepatoma FAO cells [3].

 

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