Division of Developmental Regulation
Chromosome Biology

Takemoto K., Tani N., Takada Y., Fujimura S., Tanno N., Yamane M., Okamura K., Sugimoto M., Araki K., Ishiguro K.  A meiosis-specific factor C19orf57/4930432K21Rik/BRME1 modulates localization of RAD51 and DMC1 recombinases to DSBs in mouse meiotic recombination.  Cell Reports in press (2020)


Nakatake Y, Ko BHS, Sharov A, Wakabayashi S, Murakami M, Sakota M, Chikazawa N, Ookura C, Sato S, Ito N, Ishikawa-Hirayama M, Mak SS, Jakt LM, Ueno T, Hiratsuka K, Matsushita M, Goparaju SK, Akiyama T, Ishiguro K. Oda M, Gouda N, Umezawa A, Akutsu H, Nishimura K, Matoba R, Ohara O, Ko MSH : Generation and profiling of 2,135 human ESC lines for the systematic analyses of cell states perturbed by inducing single transcription factors.  Cell Reports in press (2020)


Ishiguro K*, Matsuura K, Tani N, Takeda N, Usuki S, Yamane M,  Sugimoto M,  Fujimura S,  Hosokawa M, Chuma S,  Ko S.H.M, Araki K, Niwa H : MEIOSIN directs the switch from mitosis to meiosis in mammalian germ cells. Dev. Cell 52(4), 429-445(2020)


Preview : MEIOSIN: A New Watchman of Meiotic Initiation in Mammalian Germ Cells. Dev Cell 54, 397-398 (2020) https://www.cell.com/developmental-cell/fulltext/S1534-5807(20)30098-8

F1000Prime Recommendation   10.3410/f.737328633.793571169



Tanno N, Kuninaka S, Fujimura S, Okamura K, Takemoto K, Araki K, Araki M, Saya H,  Ishiguro K :  Phosphorylation of the Anaphase Promoting Complex activator CDH1/FZR regulates the transition from Meiosis I to Meiosis II in mouse male germ cell bioRxiv/2020/990127 (2020)

doi: https://doi.org/10.1101/2020.03.13.990127


Takemoto K., Imai Y. *(*: equally contributed), Saito K., Kawasaki T., Carlton M.P., Ishiguro K., Sakai N. : Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes. PLoS Genet 16(2): e1008640. (2020) (発生研共同利用)


Ishiguro K.  The cohesin complex in mammalian meiosis (Review) : Genes Cells. 24(1):6-30. (2019)


Hiratsuka K, Monkawa T, Akiyama T, Nakatake Y, Oda M, Goparaju SK, Kimura H, Chikazawa-Nohtomi N, Sato S, Ishiguro K., Yamaguchi S, Suzuki S, Morizane R, Ko BSH, Itoh H, Ko MSK: Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors : Scientific reports 29;9(1):913. (2019)


Ida H., Akiyama T., Ishiguro K., Goparaju SK., Nakatake Y., Chikazawa NN., Sato S., Kimura H., Yokoyama Y., Nagino M., Ko MSH., Ko SBH. : Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAsencoding transcription factors: Stem Cell Res Ther. 9, 277(2018)


Ishiguro K., Nakatake Y., Chikazawa-Nohtomi N., Kimura H., Akiyama T., Oda M., Ko SBH., Ko MSH. : Expression analysis of the endogenous Zscan4 locus and its coding proteins in mouse ES cells and preimplantation embryos: In Vitro Cell.Dev.Biol. 53, 179-190 (2017)


Ishiguro K., Monti M., Akiyama T., Kimura H., Chikazawa-Nohtomi N., Sakota M., Sato S., Redi CA., Ko SBH., Ko MSH. : Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis : In Vitro Cell.Dev.Biol. 53, 167-178 (2017)


Ishiguro K., Watanabe Y. : The cohesin REC8 prevents illegitimate inter-sister synaptonemal complex: EMBO reports 17, 783-784 (2016)


(†: equally contributed)†Kim J, †Ishiguro K., Nambu A., Akiyoshi B., Yokobayashi S., Kagami A., Ishiguro T., Pendas A.M., Takeda N., Sakakibara Y., Kitajima T.S., Tanno Y., Sakuno T., Watanabe Y. : Meikin is a conserved regulator of meiosis-I-specific kinetochore function: Nature 517, 466-471 (2015)


Kumar R., Ghyselinck N., Ishiguro K., Watanabe Y., Kouznetsova A., Höög C., Strong E., Schimenti J., Daniel K., Toth A., de Massy B.: MEI4: a central player in the regulation of meiotic DNA double strand break formation in mouse: J. Cell Science 128(9), 1800-1811 (2015)


Ishiguro K., Kim J, Shibuya H, Hernández-Hernández A, Suzuki A, Fukagawa T, Shioi G, Kiyonari H, Li XC, Schimenti J, Höög C, Watanabe Y.: Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes. Genes&Development 28, 594-607 (2014)


Shibuya H, Ishiguro K, Watanabe Y.: The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis. Nature Cell Biology 16:145-156 (2014)


Belzil C., Asada N., Ishiguro K., Nakaya T., Parsons K., Pendolino V., Neumayer G., Mapelli M., Nakatani Y., Sanada K., Nguyen MD. : p600 regulates spindle orientation in apical neural progenitors and contributes to neurogenesis in the developing neocortex. Biol.OPEN 8;3(6):475-485 (2014)


(†: equally contributed) †Nakaya T., †Ishiguro K., Belzil C., Rietsch A.M., Yu Q., Mizuno S., Bronson R.T., Geng Y., Nguyen M.D., Akashi K., Sicinski P., Nakatani Y. : p600 Plays Essential Roles in Fetal Development., Plos One 8(6): e66269 (2013)


Kim J., Ishiguro K., Kudo N., Watanabe Y. Meiosis specific cohesins in mouse embryonic oocytes. Methods in Molecular Biology 957: 47-57. (2012)


Morimoto A, Shibuya H, Zhu X, Kim J, Ishiguro K, Han M, Watanabe Y. : A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. J. Cell Biol. 198:165-172 (2012)


Ishiguro K., Kim J., Fujiyama-Nakamura S., Kato S., Watanabe Y. A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing: EMBO report 12 : 267-275 (2011)


Tanno Y., Kitajima T., Honda T., Ando Y., Ishiguro K., Watanabe Y.: Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres: Genes&Development 24 : 2169-2179 (2010)


Kawashima SA, Yamagishi Y, Honda T, Ishiguro K, Watanabe Y. :Phosphorylation of H2A by Bub1 Prevents Chromosomal Instability Through Localizing Shugoshin. Science. 327 : 172-177 (2010)


Shim SY., Wang J., Neumayer G., Tran H., Ishiguro K., Sanada K., Nakatani Y., Nguyen MD. : p600 is a Microtubule / Endoplasmic Reticulum-Associated Protein in CNS Neurons : J. Neuroscience 28 : 3604-3614 (2008)


Ishiguro K, Watanabe Y.: Chromosome cohesion in mitosis and meiosis.  J.Cell Science 120 : 367-369 (2007)


Kitajima TS, Sakuno T, Ishiguro K, Iemura S, Natsume T, Kawashima SA, Watanabe Y. :
Shugoshin collaborates with protein phosphatase 2A to protect cohesin. Nature 441 : 46-52 (2006)


Nakatani Y, Konishi H, Vassilev A, Kurooka H, Ishiguro K, Sawada J, Ikura T, Korsmeyer SJ, Qin J, Herlitz AM. : p600, a unique protein required for membrane morphogenesis and cell survival. Proc Natl Acad Sci USA.;102:15093-15098 (2005)


(†: equally contributed) Ogawa H†, Ishiguro K.†, Gaubatz S., Livingston D., Nakatani Y. : A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells, Science 296 : 1132-1136 (2002)


Nagawa F., Kodama M., Nishihara T., Ishiguro K., Sakano H.: Footprint analysis of recombination signal sequences in the 12/23 synaptic complex of V(D)J recombination, Mol. Cell. Biol. 22 : 7217-7225 (2002)


Yoshida T., Tsuboi A., Ishiguro K., Nagawa F., Sakano H.: The DNA bending protein, HMG1, is required for correct cleavage of 23bp-recombination signal sequences by RAG proteins in vitro, Int.Immunol. 12 : 101-109 : (2000)


(†: equally contributed) Nagawa F.†, Ishiguro K.†, Tsuboi A.†, Yoshida T., Ishikawa A., Takemori T., Otsuka A.J., Sakano H. : Footprint analysis of the RAG protein recombination signal sequence complex for V(D)J type recombination, Mol. Cell. Biol. 18 : 655-663(1998)