#Alavattam KG, #Esparza JM, #Hu M, #Shimada R, Kohrs AR, Abe H, Munakata Y, Otsuka K, Yoshimura S, Kitamura Y, Yeh YH, Hu YC, Kim J, Andreassen PR, ※Ishiguro K, ※Namekawa SH. (# Equally contributed, ※co-corresponding) : ATF7IP2/MCAF2 directs H3K9 methylation and meiotic gene regulation in the male germline. Genes & Development 38 (3-4) (2024)
http://www.genesdev.org/cgi/doi/10.1101/gad.351569.124
(Research Center for High Depth Omics)
Yoshimura S, Shimada R, Kikuchi K, Kawagoe S, Abe H, Iisaka S, Fujimura S, Yasunaga K, Usuki S, Tani N, Ohba T, Kondoh E, Saio T, Araki K, Ishiguro K : Atypical heat shock transcription factor HSF5 is critical for male meiotic prophase under non-stress conditions. bioRxiv (2023)
doi: https://doi.org/10.1101/2023.09.19.557986
(Research Center for High Depth Omics)
Akiyama T, Ishiguro K, Chikazawa N, Ko SBH, Yukawa M, Ko MSH : ZSCAN4-binding motif – TGCACAC is conserved and enriched in CA/TG microsatellites in both mouse and human genomes. DNA Research 31(1), dsad029 (2024)
https://doi.org/10.1093/dnares/dsad029
Shimada R, Kato Y, Takeda N, Fujimura S, Yasunaga K, Usuki S, Niwa H, Araki K, Ishiguro K : STRA8–RB interaction is required for timely entry of meiosis in mouse female germ cells. Nature Communications 14, 6443 (2023)
DOI：https://doi.org/10.1038/s41467-023-42259-6
(Research Center for High Depth Omics)
Kuwayama N, Kujirai T, Kishi Y, Hirano R, Echigoya K, Watanabe S, Nakao M, Suzuki Y, Ishiguro K, Kurumizaka H, Gotoh Y: HMGA2 directly mediates chromatin condensation in association with neuronal fate regulation. Nature Communications 14, 6420 (2023)
DOI：https://doi.org/10.1038/s41467-023-42094-9
(Joint Research Center)
Mwalilino L, Yamane M, Ishiguro K, Usuki S, Endoh M, Niwa H : The Role of Zfp352 in the regulation of transient expression of 2-cell specific genes in mouse embryonic stem cells. Genes Cells.
DOI: 10.1111/gtc.13070 (2023)
(Research Center for High Depth Omics)
Frey T, Murakami T, Maki K, Kawaue T, Tani N, Sugai A, Nakazawa N, Ishiguro KI, Adachi T, Kengaku M, Ohki K, Gotoh Y, Kishi Y. : Age-associated reduction of nuclear shape dynamics in excitatory neurons of the visual cortex. Aging Cell. e13925. (2023) DOI: 10.1111/acel.13925 (Joint Research Center, Research Center for High Depth Omics)
Shimada R, Ishiguro K : Cell cycle regulation for meiosis in mammalian germ cells. (Review) Journal of Reproduction and Development. 69 (3) 139-146 (2023)
Doi: 10.1262/jrd.2023-010
Ishihara T, Fenelon J, Griffith O, Ishiguro K, Renfree M : Conserved H3K27me3-associated chromatin remodeling allows STRA8 but not MEIOSIN expression in mammalian germ cells. Reproduction 165, 507-520 (2023) Doi: 10.1530/REP-22-0286 (Research Center for High Depth Omics, Internship)
Ito T, Ohta M, Osada A, Nishiyama A, Ishiguro KI, Tamura T, Sekita Y, Kimura
T. Switching defective/sucrose non-fermenting chromatin remodeling complex
coordinates meiotic gene activation via promoter remodeling and Meiosin
activation in female germline. Genes Cells. Nov 12. doi: 10.1111/gtc.12990. (2022)
https://doi.org/10.1111/gtc.12990
Abe H, Yeh YH, Munakata Y, Ishiguro KI, Andreassen PR, Namekawa SH. : Active DNA damage response signaling initiates and maintains meiotic sex chromosome inactivation. Nat Commun. 13(1):7212. (2022)
doi:10.1038/s41467-022-34295-5.
Yamanaka S, Namekawa S, Ishiguro K. : Editorial: Regulatory mechanisms of gene expression, chromatin structure and nuclear dynamics in gametogenesis. Front Cell Dev Biol. 10, 995650. (2022)
DOI: 10.3389/fcell.2022.995650
Tani N, Tanno N, Ishiguro K. : Tandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis. STAR Protocols 3 (2), 10145 (2022)
https://doi.org/10.1016/j.xpro.2022.101452 (Research Center for High Depth Omics)
Sano H, Nakamura A, Yamane M, Niwa H, Nishimura T, Araki K, Takemoto K, Ishiguro K, Aoki H, Kato Y, Kojima M : The polyol pathway is an evolutionarily conserved system for sensing glucose uptake. PLOS Biology 20(6): e3001678. (2022)
https://doi.org/10.1371/journal.pbio.3001678 (Joint Research Center, Research Center for High Depth Omics)
Oura S, Hino T, Satoh T, Noda T, Koyano T, sotani A, Matsuyama M, Akira S, Ishiguro K, Ikawa M : Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice. PLOS Genetics 18(6): e1010241 (2022)
DOI:10.1371/journal.pgen.1010241
Hirano K, Nonami Y, Nakamura Y, Sato T, Sato T, Ishiguro K, Ogawa T, Yoshida S. : Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis. Communications Biology 5, 504. (2022)
DOI: 10.1038/s42003-022-03449-y
Saito Y, Santosa V, Ishiguro K, Kanemaki M : MCMBP promotes the assembly of the MCM2–7 hetero-hexamer to ensure robust DNA replication in human cells. eLife. 11, e77393 (2022) https://elifesciences.org/articles/77393
Kikuchi K, Sakamoto Y, Uezu A, Yamamoto H, Ishiguro K, Shimamura K, Saito T, Hisanaga S, Nakanishi H. : Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms to control cell motility and neurite outgrowth. Life Science Alliance (2022) in press
*Ishiguro K. : Mechanism of initiation of meiosis in mouse germ cells (Review)
Current Topics in Developmental Biology 151(2022)
DOI:10.1016/bs.ctdb.2022.04.005
Tanno N, Takemoto K, Takada-Horisawa Y, Shimada R., Fujimura S, Tani N., Takeda N., Araki K, *Ishiguro K : FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis. iScience 25(4),104008 (2022)
https://doi.org/10.1016/j.isci.2022.104008
*Ishiguro K. : Sexually dimorphic properties in meiotic chromosome. (Review) Sexual Development (2021) doi:10.1159/000520682
*Ishiguro K, Shimada R. : MEIOSIN directs initiation of meiosis and subsequent meiotic prophase programs during spermatogenesis. (Review) Genes and Genetic Systems (2021) in press
Takeda I, Araki M, Ishiguro K, Ohga T, Takada K, Yamaguchi Y, Hashimoto K, Kai T, Yoshinobu K, Nakagata N, Imasaka M, Araki K.
Gene trapping reveals a new transcriptionally active genome element: the chromosome-specific clustered trap region. Genes Cells.26, 874-890 (2021) DOI: 10.1111/gtc.12890
Horisawa-Takada Y, Kodera C, Takemoto K, Sakashita A, Horisawa K, Maeda R, Shimada R, Usuki S, Fujimura S, Tani N, Matsuura K, Akiyama T, Suzuki A, Niwa H, Tachibana M, Ohba T, Katabuchi H, Namekawa S, Araki K, Ishiguro K. Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis
Nature Communications 12: (2021) DOI : 10.1038/s41467-021-23378-4
Takada Y, Yaman-Deveci R, Shirakawa T, Sharif J, Tomizawa S, Miura F, Ito T, Ono M, Nakajima K, Koseki Y, Shiotani F, Ishiguro K, Ohbo K, Koseki H. Maintenance DNA methylation in pre-meiotic germ cells regulates meiotic prophase by facilitating homologous chromosome pairing .Development 148(10):dev194605. (2021)
Oura S, Koyano T, Kodera C, Takada Y, Matsuyama M, Ishiguro K, Ikawa M. KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. PLOS Genetics 17(5): e1009412 (2021)
Fujiwara Y., Horisawa-Takada Y., Inoue E., Tani N., Shibuya H., Fujimura S., Kariyazono R., Sakata T., Ohta K., Araki K., Okada Y., Ishiguro K. Meiotic cohesins mediate initial loading of HORMAD1 to the chromosomes and coordinate SC formation during meiotic prophase. PLOS Genetics 16(9): e1009048. (2020)
Tanno N, Kuninaka S, Fujimura S, Takemoto K, Okamura K, Takeda N, Araki K, Araki M, Saya H, Ishiguro K* : Phosphorylation of the Anaphase Promoting Complex activator FZR1/CDH1 is required for Meiosis II entry in mouse male germ cell. Scientific Reports in press (2020)
https://doi.org/10.1038/s41598-020-67116-0
Takemoto K., Tani N., Takada Y., Fujimura S., Tanno N., Yamane M., Okamura K., Sugimoto M., Araki K., Ishiguro K. Meiosis-specific C19orf57/4930432K21Rik/BRME1 modulates localization of RAD51 and DMC1 to DSBs in mouse meiotic recombination. Cell Reports 31, 107686 (2020)
https://doi.org/10.1016/j.celrep.2020.107686
Nakatake Y, Ko BHS, Sharov A, Wakabayashi S, Murakami M, Sakota M, Chikazawa N, Ookura C, Sato S, Ito N, Ishikawa-Hirayama M, Mak SS, Jakt LM, Ueno T, Hiratsuka K, Matsushita M, Goparaju SK, Akiyama T, Ishiguro K. Oda M, Gouda N, Umezawa A, Akutsu H, Nishimura K, Matoba R, Ohara O, Ko MSH : Generation and profiling of 2,135 human ESC lines for the systematic analyses of cell states perturbed by inducing single transcription factors. Cell Reports in press (2020)
Ishiguro K*, Matsuura K, Tani N, Takeda N, Usuki S, Yamane M, Sugimoto M, Fujimura S, Hosokawa M, Chuma S, Ko S.H.M, Araki K, Niwa H : MEIOSIN directs the switch from mitosis to meiosis in mammalian germ cells. Dev. Cell 52(4), 429-445(2020)
https://www.cell.com/developmental-cell/fulltext/S1534-5807(20)30011-3
Preview : MEIOSIN: A New Watchman of Meiotic Initiation in Mammalian Germ Cells. Dev Cell 54, 397-398 (2020) https://www.cell.com/developmental-cell/fulltext/S1534-5807(20)30098-8
F1000Prime Recommendation 10.3410/f.737328633.793571169
Takemoto K., Imai Y. *(*: equally contributed), Saito K., Kawasaki T., Carlton M.P., Ishiguro K., Sakai N. : Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes. PLoS Genet 16(2): e1008640. (2020) (発生研共同利用)
Ishiguro K. The cohesin complex in mammalian meiosis (Review) : Genes Cells. 24(1):6-30. (2019)
Hiratsuka K, Monkawa T, Akiyama T, Nakatake Y, Oda M, Goparaju SK, Kimura H, Chikazawa-Nohtomi N, Sato S, Ishiguro K., Yamaguchi S, Suzuki S, Morizane R, Ko BSH, Itoh H, Ko MSK: Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors : Scientific reports 29;9(1):913. (2019)
Ida H., Akiyama T., Ishiguro K., Goparaju SK., Nakatake Y., Chikazawa NN., Sato S., Kimura H., Yokoyama Y., Nagino M., Ko MSH., Ko SBH. : Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAsencoding transcription factors: Stem Cell Res Ther. 9, 277(2018)
Ishiguro K., Nakatake Y., Chikazawa-Nohtomi N., Kimura H., Akiyama T., Oda M., Ko SBH., Ko MSH. : Expression analysis of the endogenous Zscan4 locus and its coding proteins in mouse ES cells and preimplantation embryos: In Vitro Cell.Dev.Biol. 53, 179-190 (2017)
Ishiguro K., Monti M., Akiyama T., Kimura H., Chikazawa-Nohtomi N., Sakota M., Sato S., Redi CA., Ko SBH., Ko MSH. : Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis : In Vitro Cell.Dev.Biol. 53, 167-178 (2017)
Ishiguro K., Watanabe Y. : The cohesin REC8 prevents illegitimate inter-sister synaptonemal complex: EMBO reports 17, 783-784 (2016)
(†: equally contributed)†Kim J, †Ishiguro K., Nambu A., Akiyoshi B., Yokobayashi S., Kagami A., Ishiguro T., Pendas A.M., Takeda N., Sakakibara Y., Kitajima T.S., Tanno Y., Sakuno T., Watanabe Y. : Meikin is a conserved regulator of meiosis-I-specific kinetochore function: Nature 517, 466-471 (2015)
Kumar R., Ghyselinck N., Ishiguro K., Watanabe Y., Kouznetsova A., Höög C., Strong E., Schimenti J., Daniel K., Toth A., de Massy B.: MEI4: a central player in the regulation of meiotic DNA double strand break formation in mouse: J. Cell Science 128(9), 1800-1811 (2015)
Ishiguro K., Kim J, Shibuya H, Hernández-Hernández A, Suzuki A, Fukagawa T, Shioi G, Kiyonari H, Li XC, Schimenti J, Höög C, Watanabe Y.: Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes. Genes&Development 28, 594-607 (2014)
Shibuya H, Ishiguro K, Watanabe Y.: The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis. Nature Cell Biology 16:145-156 (2014)
Belzil C., Asada N., Ishiguro K., Nakaya T., Parsons K., Pendolino V., Neumayer G., Mapelli M., Nakatani Y., Sanada K., Nguyen MD. : p600 regulates spindle orientation in apical neural progenitors and contributes to neurogenesis in the developing neocortex. Biol.OPEN 8;3(6):475-485 (2014)
(†: equally contributed) †Nakaya T., †Ishiguro K., Belzil C., Rietsch A.M., Yu Q., Mizuno S., Bronson R.T., Geng Y., Nguyen M.D., Akashi K., Sicinski P., Nakatani Y. : p600 Plays Essential Roles in Fetal Development., Plos One 8(6): e66269 (2013)
Kim J., Ishiguro K., Kudo N., Watanabe Y. Meiosis specific cohesins in mouse embryonic oocytes. Methods in Molecular Biology 957: 47-57. (2012)
Morimoto A, Shibuya H, Zhu X, Kim J, Ishiguro K, Han M, Watanabe Y. : A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. J. Cell Biol. 198:165-172 (2012)
Ishiguro K., Kim J., Fujiyama-Nakamura S., Kato S., Watanabe Y. A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing: EMBO report 12 : 267-275 (2011)
Tanno Y., Kitajima T., Honda T., Ando Y., Ishiguro K., Watanabe Y.: Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres: Genes&Development 24 : 2169-2179 (2010)
Kawashima SA, Yamagishi Y, Honda T, Ishiguro K, Watanabe Y. :Phosphorylation of H2A by Bub1 Prevents Chromosomal Instability Through Localizing Shugoshin. Science. 327 : 172-177 (2010)
Shim SY., Wang J., Neumayer G., Tran H., Ishiguro K., Sanada K., Nakatani Y., Nguyen MD. : p600 is a Microtubule / Endoplasmic Reticulum-Associated Protein in CNS Neurons : J. Neuroscience 28 : 3604-3614 (2008)
Ishiguro K, Watanabe Y.: Chromosome cohesion in mitosis and meiosis. J.Cell Science 120 : 367-369 (2007)
Kitajima TS, Sakuno T, Ishiguro K, Iemura S, Natsume T, Kawashima SA, Watanabe Y. :
Shugoshin collaborates with protein phosphatase 2A to protect cohesin. Nature 441 : 46-52 (2006)
Nakatani Y, Konishi H, Vassilev A, Kurooka H, Ishiguro K, Sawada J, Ikura T, Korsmeyer SJ, Qin J, Herlitz AM. : p600, a unique protein required for membrane morphogenesis and cell survival. Proc Natl Acad Sci USA.;102:15093-15098 (2005)
(†: equally contributed) Ogawa H†, Ishiguro K.†, Gaubatz S., Livingston D., Nakatani Y. : A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells, Science 296 : 1132-1136 (2002)
Nagawa F., Kodama M., Nishihara T., Ishiguro K., Sakano H.: Footprint analysis of recombination signal sequences in the 12/23 synaptic complex of V(D)J recombination, Mol. Cell. Biol. 22 : 7217-7225 (2002)
Yoshida T., Tsuboi A., Ishiguro K., Nagawa F., Sakano H.: The DNA bending protein, HMG1, is required for correct cleavage of 23bp-recombination signal sequences by RAG proteins in vitro, Int.Immunol. 12 : 101-109 : (2000)
(†: equally contributed) Nagawa F.†, Ishiguro K.†, Tsuboi A.†, Yoshida T., Ishikawa A., Takemori T., Otsuka A.J., Sakano H. : Footprint analysis of the RAG protein recombination signal sequence complex for V(D)J type recombination, Mol. Cell. Biol. 18 : 655-663(1998)
