Division of Developmental Regulation
Medical Cell Biology
Publications

PRESENT-1998 (selected) :

 

 

Y. Hino, K. Nagaoka, S. Oki, K. Etoh, S. Hino, and M. Nakao. Mitochondrial stress induces AREG expression and epigenomic remodeling through c-JUN and YAP-mediated enhancer activation. Nucleic Acids Res. (in press).

 

U. Thamrongwaranggoon, M. Detarya, W. Seubwai, C. Saengboonee, S. Hino, T. Koga, M. Nakao, and S. Wongkham. Lactic acidosis promotes aggressive features of cholangiocarcinoma cells via upregulating ALDH1A3 expression through EGFR axis. Life Sci. 302: 120648, 2022.

 

S. Hino, T. Sato, and M. Nakao. Chromatin immunoprecipitation sequencing for detecting histone modifications and modifiers. Methods Mol. Biol., Springer (in press).

 

Y. Hayashi, S. Kashio, K. Murotomi, S. Hino, W. Kang, K. Miyado, M. Nakao, M. Miura, S. Kobayashi, and M. Namihira. Biosynthesis of S-adenosyl-methionine enhances aging-related defects in Drosophila oogenesis. Sci. Rep. 12: 5593, 2022.

 

M. Kusakabe, E. Kakumu, F. Kurihara, K. Tsuchida, T. Maeda, H. Tada, K. Kusao, A. Kato, T. Yasuda, T. Matsuda, M. Nakao, M. Yokoi, W. Sakai, and K. Sugasawa. Histone deacetylation regulates nucleotide excision repair through an interaction with the XPC protein. iScience 25: 104040, 2022.

 

H. Matsumori, K. Watanabe, H. Tachiwana, T. Fujita, Y. Ito, M. Tokunaga, K. Sakata-Sogawa, H. Osakada, T. Haraguchi, A. Awatsu, H. Ochiai, Y. Sakata, K. Ochiai, T. Toki, E. Ito, I.G. Goldberg, K. Tokunaga, M. Nakao, and N. Saitoh. Ribosomal protein L5 facilitates rDNA bundling and nucleolar assembly. Life Sci. Alliance 5: e202101045, 2022.

 

T. Igata, H. Tanaka, K. Etoh, S. Hong, N. Tani, T. Koga, and M. Nakao. Loss of the transcription repressor ZHX3 induces senescence-associated gene expression and mitochondrial-nucleolar activation. PLoS One 17: e0262488, 2022.

 

Y. Kanki, M. Muramatsu, Y. Miyamura, K. Kikuchi, Y. Higashijima, R. Nakaki, JI. Suehiro, Y. Sasaki, Y. Kubota, H. Koseki, H. Morioka, T. Kodama, M. Nakao, D. Kurotaki, H. Aburatani, and T. Minami. Bivalent-histone-marked immediate-early gene regulation is vital for VEGF-responsive angiogenesis. Cell Rep. 38: 110332, 2022.

 

Kajioka, K. Suzuki, S. Matsushita, S. Hino, T. Sato, S. Takada, K. Isono, T. Takeo, M. Kajimoto, N. Nakagata, M. Nakao, M. Suyama, T. DeFalco, S. Miyagawa, and G. Yamada. Sexual fate of murine external genitalia development: Conserved transcriptional competency for male-biased genes in both sexes. Proc. Natl. Acad. Sci. USA. 118: e2024067118, 2021.

 

K. Kohrogi, S. Hino, A. Sakamoto, K. Anan, R. Takase, H. Araki, Y. Hino, K. Araki, T. Sato, K. Nakamura, and M. Nakao. LSD1 defines erythroleukemia metabolism by controlling lineage-specific transcription factors GATA1 and C/EBPα. Blood Adv.  5: 2305-2318, 2021.

 

H. Ohguchi, P. Park, T. Wang, B. Gryder, D. Ogiya, K. Kurata, X. Zhang, D. Li, C. Pei, T. Masuda, C. Johansson, V. Wimalasena, Y. Kim, S. Hino, S. Usuki, Y. Kawano, M. Samur, Y-T. Tai, N. Munshi, M. Matsuoka, S. Ohtsuki, M. Nakao, T. Minami, S. Lauberth, J. Khan, U. Oppermann, A. Durbin, K. Anderson, T. Hideshima, and J. Qi. Lysine demethylase 5A is required for MYC driven transcription in multiple myeloma. Blood Cancer Discov.  2: 370-387, 2021.

 

Y. Arima, Y. Nakagawa, T. Takeo, T. Ishida, T. Yamada, S. Hino, M. Nakao, S. Hanada, T. Umemoto, T. Suda, T. Sakuma, T. Yamamoto, T. Watanabe, K. Nagaoka, Y. Tanaka, Y.K. Kawamura, K. Tonami, H. Kurihara, Y. Sato, K. Yamagata, T. Nakamura, S. Araki, E. Yamamoto, Y. Izumiya, K. Sakamoto, K. Kaikita, K. Matsushita, K. Nishiyama, N. Nakagata, and K. Tsujita. Murine neonatal ketogenesis preserves mitochondrial energetics by preventing protein hyperacetylation. Nature Metab. 3: 196-210, 2021.

 

U. Tonsri, S. Wongkham, C. Wongkham, T. Koga, S. Hino, M. Nakao, S. Roytrakul, and W. Seubwai. High glucose-oxidative condition enhances proliferation and migration in cholangiocarcinoma cells via upregulation of alpha mannosidase MAN2A2 and chromatin factor CHD8. Cancer Sci. 112: 254-264, 2021.

 

T. Koga, F. Sasaki, K. Saeki, S. Tsuchiya, T. Okuno, M. Ohba, T. Ichiki, S. Iwamoto, H. Uzawa, K. Kitajima, C. Meno, E. Nakamura, N. Tada, Y. Fukui, J. Kikuta, M. Ishii, Y. Sugimoto, M. Nakao, and T. Yokomizo. Expression of leukotriene B4 receptor 1 defines functionally distinct DCs that control allergic skin inflammation. Cell. Mol. Immunol. 18: 1437-1449, 2021.

 

M. Nakao, H. Tanaka and T. Koga. Cellular senescence variation by metabolic and epigenomic remodeling. Trends Cell Biol. 30: 919-922, 2020.

 

T. Koga, F. Sasaki, K. Saeki, S. Tsuchiya, T. Okuno, M. Ohba, T. Ichiki, S. Iwamoto, H. Uzawa, K. Kitajima, C. Meno, E. Nakamura, N. Tada, Y. Fukui, J. Kikuta, M. Ishii, Y. Sugimoto, M. Nakao, T. Yokomizo. Expression of leukotriene B4 receptor 1 defines functionally distinct DCs that control allergic skin inflammation. Cell. Mol. Immunol. (in press)

 

H. Tanaka, T. Igata, K. Etoh, T. Koga, S. Takebayashi, and M. Nakao. The NSD2/WHSC1/MMSET methyltransferase protects cellular senescence-associated epigenomic remodeling. Aging Cell 19: e13173, 2020.

 

T. Yamamoto, A. Hirosue, M. Nakamoto, R. Yoshida, J. Sakata, Y. Matsuoka, K. Kawahara, Y. Nagao, M. Nagata, N. Takahashi, A. Hiraki, M. Shinohara, M. Nakao, N. Saitoh and H. Nakayama. BRD4 promotes metastatic potential in oral squamous cell carcinoma through the epigenetic regulation of the MMP2 gene. Br. J. Cancer 123: 580-590, 2020.

 

J. Tamaoki, M. Takeuchi, R. Abe, H. Kaneko, T. Wada, S. Hino, M. Nakao, Y. Furukawa, and M. Kobayashi. Splicing- and demethylase-independent functions of LSD1 in zebrafish primitive hematopoiesis. Sci. Rep. 10: 8521, 2020.

 

T. Horii, S. Morita, S. Hino, M. Kimura, Y. Hino, H. Kogo, M. Nakao, and I. Hatada. Successful generation of epigenetic disease model mice by targeted demethylation of the epigenome. Genome Biol. 21: 77, 2020.

 

Y. Yasuda, K. Tokunaga, T. Koga, C. Sakamoto, I.G. Goldberg, N. Saitoh, and M. Nakao. Computational analysis of morphological and molecular features in gastric cancer tissues. Cancer Med. 9: 2223-2234, 2020.

 

R. Fujita, T. Yamamoto, Y. Arimura, S. Fujiwara, H. Tachiwana, Y. Ichikawa, Y. Sakata, L. Yang, R. Maruyama, M. Hamada, M. Nakao, N. Saitoh, and H. Kurumizaka. Nucleosome destabilization by nuclear-RNAs. Commun. Biol. 3: 60, 2020.

 

N. Nishikura, K. Hino, T. Kimura, Y. Uchimura, S. Hino, M. Nakao, Y. Maruo, and J. Udagawa. Postweaning iron deficiency in male rats leads to long-term hyperactivity and decreased Reelin gene expression in the nucleus accumbens. J. Nutrition 150: 212-221, 2020.

 

T. Osawa, T. Shimamura, K. Saito, Y. Hasegawa, N. Ishii, M. Nishida, R. Ando, A. Kondo, M. Anwar, R. Tsuchida, S. Hino, A. Sakamoto, K. Igarashi, K. Saitoh, K. Kato, K. Endo, S. Yamano, Y. Kanki, Y. Matsumura, T. Minami, T. Tanaka, M. Anai, Y. Wada, H. Wanibuchi, M. Hayashi, A. Hamada, M. Yoshida, S. Yachida, M. Nakao, J. Sakai, H. Aburatani, M. Shibuya, K. Hanada, S. Miyano, T. Soga, and T. Kodama. Phosphoethanolamine Accumulation Protects Cancer Cells under Glutamine Starvation through Downregulation of PCYT2. Cell Rep. 29: 89-103, 2019.

 

M.O.A. Abdalla, T. Yamamoto, K. Maehara, J. Nogami, Y. Ohkawa, H. Miura, R. Poonperm, I. Hiratani, H. Nakayama, M. Nakao, and N. Saitoh. The Eleanor ncRNAs activate the topological domain of the ESR1 locus to balance against apoptosis. Nature Commun. 10: 3778, 2019.

 

K. Nishimura, Y. Cho, K. Tokunaga, M. Nakao, T. Tani, and T. Ideue. DEAH box RNA helicase DHX38 associates with satellite I noncoding RNA involved in chromosome segregation. Genes Cells 24: 585-590, 2019.

 

M. Nakao, K. Anan, H. Araki, and S. Hino. Distinct roles of NAD+-Sirt1 and FAD-LSD1 pathways in metabolic response and tissue development. Trends Endocrinol. Metab. 30: 409-412, 2019.

 

A.R. Acebedo, K. Suzuki, S. Hino, M.C. Alcantara, H. Haga, K. Matsumoto, M. Nakao, K. Shimamura, T. Taeko, N. Nakagata, S. Miyagawa, R. Nishinakamura, R.S Adelstein, and G. Yamada. Mesenchymal actomyosin contractility is required for androgen-driven mouse urethral masculinization. Commun. Biol. 2: 95, 2019.

 

R. Takase, S. Hino, K. Nagaoka, K. Anan, K. Kohrogi, H. Araki, Y. Hino, A. Sakamoto, T. B. Nicholson, T. Chen and M. Nakao. Lysine-specific demethylase-2 is distinctively involved in brown and beige adipogenic differentiation. FASEB J. 33: 5300-5311, 2019.

 

T. Yamamoto, C. Sakamoto, H. Tachiwana, M. Kumabe, T. Matsui, T. Yamashita, M. Shinagawa, K. Ochiai, N. Saitoh, and M. Nakao. Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA. Sci. Rep. 8: 15202, 2018.

 

M. Nakao, S. Fujiwara, and H. Iwase. Cancer navigation strategy for endocrine therapy-resistant breast tumors. Trends Cancer 4: 404-407, 2018.

 

K. Anan, S. Hino, N. Shimizu, A. Sakamoto, K. Nagaoka, R. Takase, K. Kohrogi, H. Araki, Y. Hino, S. Usuki, S. Oki, H. Tanaka, K. Nakamura, F. Endo, and M. Nakao. LSD1 mediates metabolic reprogramming by glucocorticoids during myogenic differentiation. Nucleic Acids Res. 46: 5441-5454, 2018.

 

M. Takagi, T. Ono, T. Natsume, C. Sakamoto, M. Nakao, N. Saitoh, M.T. Kanemaki, T. Hirano, and N. Imamoto. Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms. J. Cell Sci. 131: 1-13, 2018.

 

T. Kimura, K. Hino, T. Kono, A. Takano, N. Nitta, N. Ushio, S. Hino, R. Takase, M. Kudo, Y. Daigo, W. Morita, M. Nakao, M. Nakatsukasa, T. Tamagawa, and J. Udagawa. Maternal undernutrition during early pregnancy in rats inhibits postnatal growth of hindlimb bones in the offspring by alteration of chondrogenesis. Gen. Comp. Endocrinol. 260: 58-66, 2018.

 

Y. Kitano, Y. Baba, S. Nakagawa, K. Miyake, M. Iwatsuki, Y. Sakamoto, Y. Yamashita, N. Yoshida, M. Watanabe, M. Nakao, and H. Baba. Nrf2 promotes esophageal cancer cell proliferation via metabolic reprogramming and ROS detoxification. J. Pathol. 244: 346-357, 2018.

 

T. Ono, C. Sakamoto, M. Nakao, N. Saitoh, and T. Hirano. Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ. Mol. Biol. Cell 28: 2875-2886, 2017.

 

A. Tanaka, M.O. Radwan, A. Hamasaki, A. Ejima, E. Obata, R. Koga, H. Tateishi, Y. Okamoto, M. Fujita, M. Nakao, K. Umezawa, F. Tamanoi, and M. Otsuka. A novel inhibitor of farnesyltransferase with a zinc site recognition moiety and a farnesyl group. Bioorg. Med. Chem. 27: 3862-3866, 2017.

 

W.A. Hassan, S. Takebayashi, M.O.A. Abdalla, K. Fujino, S. Kudoh, Y. Motooka, Y. Sato, Y. Naito, K. Higaki, J. Wakimoto, S. Okada, M. Nakao, Y. Ishikawa, and T. Ito. Correlation between histone acetylation and expression of Notch1 gene in human lung carcinoma and its role in the origin of combined small cell lung carcinoma. Lab. Invest. 97: 913-921, 2017.

 

X. Wang, S. Liang, Y. Sun, H. Li, F. Endo, M. Nakao, N. Saitoh, and L. Wu. Analysis of estrogen receptor b gene methylation in autistic males in a Chinese Han population. Metab. Brain Dis. 32: 1033-1042, 2017.

 

H. Tanaka, S. Takebayashi, A. Sakamoto, T. Igata, Y. Nakatsu, N. Saitoh, S. Hino, and M. Nakao. The loss of SETD8/PR-Set7 methyltransferase induces cellular senescence via nucleolar-mitochondrial coactivation. Cell Rep. 18: 2148-2161, 2017.

 

M. Nakamoto, K. Ishihara, T. Watanabe, A. Hirosue, S. Hino, M. Shinohara, H. Nakayama, and M. Nakao. The glucocorticoid receptor regulates the ANGPTL4 gene in a CTCF-mediated chromatin context in human hepatic cells. PLoS One 12: e0169225, 2017

 

K. Ishihara, M. Nakamoto, and M. Nakao. DNA methylation-independent removable insulator controls chromatin remodeling at the HOXA gene locus via retinoic acid signaling. Hum. Mol. Genet. 25: 5383-5394, 2016.

 

Tsutsumi, K. Iwao, H. Hayashi, T. Kawaji, T. Inoue, S. Hino, M. Nakao, and H. Tanihara. Potential neuroprotective effects of an LSD1 inhibitor in retinal ganglion cells via p38 MAPKγ activity. Invest. Ophthalmol. Vis. Sci. 57: 6461-6473, 2016.

 

S. Tomita, M.O. Abdalla, S. Fujiwara, T. Yamamoto, H. Iwase, M. Nakao, and N. Saitoh. Roles of long non-coding RNAs in chromosome domains. Wiley Interdiscip. Rev. RNA, (Reviews) 8: 2017 (doi: 10.1002/wrna.1384).

 

S. Hino, K. Kohrogi, and M. Nakao. Histone demethylase LSD1 controls the phenotypic plasticity of cancer cells. Cancer Sci. (Reviews) 107: 1187-1192, 2016.

 

P. Miyazato, H. Katsuya, A. Fukuda, Y. Uchiyama, M. Matsuo, M. Tokunaga, S. Hino, M. Nakao, and Y. Satou. Application of targeted enrichment to next-generation sequencing of retroviruses integrated into the host human genome. Sci. Rep. 6: 28324, 2016.

 

K. Nakamura, Y. Baba, K. Kosumi, K. Harada, H. Shigaki, K. Miyake, Y. Kiyozumi, M. Ohuchi, J. Kurashige, T. Ishimoto, M. Iwatsuki, Y. Sakamoto, N. Yoshida, M. Watanabe, M. Nakao, and H. Baba. UHRF1 regulates global DNA hypomethylation and is associated with poor prognosis in esophageal squamous cell carcinoma. Oncotarget 7: 57821-57831, 2016.

 

T. Nakayama, N. Saitoh, K. Morotomi-Yano, KI. Yano, M. Nakao, and H. Saitoh. Tunicamycin induces discharge of the nucleus and chromatin fibers to extracellular spaces in a range of myeloid cell lines. Cell Biol. Int. 40: 597-602, 2016.

 

Y. Satou, P. Miyazato, K. Ishihara, A. Fukuda, K. Nosaka, T. Watanabe, A. Rowan, M. Nakao, and C. R.M. Bangham. The human retrovirus HTLV-1 inserts an ectopic CTCF-binding site into the human genome. Proc. Natl. Acad. Sci. USA. 113: 3054-3059, 2016.

 

Y. Xi, W. Shen, L. Ma, M. Zhao, J. Zheng, S. Bu, S. Hino, and M. Nakao. HMGA2 promotes adipogenesis by activating C/EBPβ-mediated expression of PPARγ. Biochem. Biophys. Res. Commun. 472: 617-623, 2016.

 

A. Matsumoto, C. Sakamoto, H. Matsumori, J. Katahira, Y. Yasuda, K. Yoshidome, M. Tsujimoto, I.G. Goldberg, N. Matsuura, M. Nakao, N. Saitoh, and M. Hieda. Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli. Nucleus 7: 68-83, 2016.

 

K. Kosumi, Y. Baba, T. Ishimoto, A. Sakamoto, K. Harada, J. Kurashige, Y. Hiyoshi, M. Iwatsuki, S. Iwagami, Y. Miyamoto, Y. Sakamoto, N. Yoshida, M. Watanabe, S. Hino, M. Nakao, and H. Baba. Lysine-specific demethylase-1 contributes to malignant behavior by regulation of invasive activity and metabolic shift in esophageal cancer. Int. J. Cancer 138: 428-439, 2016.

 

Y. Matsumura, R. Nakaki, T. Inagaki, A. Yoshida, Y. Kano, H. Kimura, T. Tanaka, S. Tsutsumi, M. Nakao, T. Doi, K. Fukami, T.F. Osborne, T. Kodama, H. Aburatani, and J. Sakai. H3K4/H3K9me3 bivalent chromatin domains targeted by lineage-specific DNA methylation pauses adipocyte differentiation. Mol. Cell 60: 584-596, 2015.

 

T. Koga, M.A. Suico, S. Shimasaki, E. Watanabe, Y. Kai, K. Koyama, K. Omachi, S. Morino-Koga, T. Sato, T. Shuto, K. Mori, S. Hino, M. Nakao, and H. Kai. Endoplasmic reticulum (ER) stress induces Sirtuin 1 (SIRT1) expression via PI3K-Akt-GSK3 signaling pathway and promotes hepatocellular injury. J. Biol. Chem. 290: 30366-30374, 2015.

 

TF. Ali, K. Iwamaru, HI. Ciftci, R. Koga, M. Matsumoto, Y. Oba, H. Kurosaki, M. Fujita, Y. Okamoto, K. Umezawa, M. Nakao, T. Hide, K. Makino, J. Kuratsu, M. Abdel-Aziz, Gel-D. Abuo-Rahma, EA. Beshr, and M. Otsuka. Novel metal chelating molecules with anticancer activity. Striking effect of the imidazole substitution of the histidine-pyridine-histidine system. Bioorg. Med. Chem. 23: 5476-5482, 2015.

 

H. Kitamura, H. Matsumori, A. Kalendova, P. Hozak, I.G. Goldberg, M. Nakao, N. Saitoh, and M. Harata. The actin family protein ARP6 contributes to the structure and the function of the nucleolus. Biochem. Biophys. Res. Commun. 464: 554-560, 2015.

 

K. Wagatsuma, S. Tani-ichi, B. Liang, S. Shitara, K. Ishihara, M. Abe, H. Miyachi, S. Kitano, T. Hara, M. Nanno, H. Ishikawa, K. Sakimura, M. Nakao, H. Kimura, and K. Ikuta. STAT5 orchestrates local epigenetic changes for chromatin accessibility and rearrangements by direct binding to the TCRg locus. J. Immunol. 195: 1804-1814, 2015.

 

A. Murata, Y. Baba, T. Ishimoto, K. Miyake, K. Kosumi, K. Harada, J. Kurashige, S. Iwagami, Y. Sakamoto, Y. Miyamoto, N. Yoshida, M. Yamamoto, S. Oda, M. Watanabe, M. Nakao, and H. Baba. TET family proteins and 5-hydroxymethylcytosine in esophageal squamous cell carcinoma. Oncotarget 6: 23372-23382, 2015.

 

S. Takebayashi, H. Tanaka, S. Hino, Y. Nakatsu, T. Igata, A. Sakamoto, M. Narita, and M. Nakao. Retinoblastoma protein promotes oxidative phosphorylation through up-regulation of glycolytic genes in oncogene-induced senescent cells. Aging Cell 14: 689-697, 2015.

 

S. Tomita, M.O. Abdalla, S. Fujiwara, H. Matsumori, K. Maehara, Y. Ohkawa, H. Iwase, N. Saitoh, and M. Nakao. A cluster of non-coding RNAs activates the ESR1 locus during breast cancer adaptation to hormone deprivation. Nature Commun. 6: 6966, 2015.

 

A. Sakamoto, S. Hino, K. Nagaoka, K. Anan, R. Takase, H. Matsumori, H. Ojima, Y. Kanai, K. Arita, and M. Nakao. Lysine demethylase LSD1 coordinates glycolytic and mitochondrial metabolism in hepatocellular carcinoma cells. Cancer Res. 75: 1445-1456, 2015.

 

K. Nagaoka, S. Hino, A. Sakamoto, K. Anan, R. Takase, T. Umehara, S. Yokoyama, Y. Sasaki, and M. Nakao. Lysine-specific demethylase LSD2 suppresses lipid influx and metabolism in hepatic cells. Mol. Cell. Biol. 35: 1068-1080, 2015.

 

K. Tokunaga, N. Saitoh, I.G. Goldberg, C. Sakamoto, Y. Yasuda, Y. Yoshida, S. Yamanaka, and M. Nakao. Computational image analysis of colony and nuclear morphology to evaluate human induced pluripotent stem cells. Sci. Rep. 4: 6996, 2014.

 

S. Kanda, T. Ohmori, A. Taguchi, K. Kudo, T. Horiuchi, Y. Sato, S. Hino, Y. Suzuki, M. Sander, S. Sugano, M. Nakao, and R. Nishinakamura. Sall1 co-operates with Six2 to actively maintain nephron progenitors. J. Am. Soc. Nephrol. 25: 2584-2595, 2014.

 

N. Sugeno, T. Hasegawa, N. Tanaka, M. Fukuda, K. Wakabayashi, R. Oshima, M. Konno, E. Miura, A. Kikuchi, T. Baba, T. Anan, M. Nakao, S. Geisler, M. Aoki, and A. Takeda. K63-linked ubiquitination by Nedd4-1 facilitates endosomal sequestration of internalized alpha-synuclein. J. Biol. Chem. 289: 18137-18151, 2014.

 

T. Baba, H. Otake, T. Sato, K. Miyabayashi, Y. Shishido, C-Y. Wang, Y. Shima, H. Kimura, M. Yagi, Y. Ishihara, S. Hino, H. Ogawa, M. Nakao, T. Yamazaki, D. Kang, Y. Ohkawa, M. Suyama, B-C. Chung, and K. Morohashi. Glycolytic genes as the targets of a nuclear receptor Ad4BP/SF-1. Nature Commun. 5: 3634, 2014.

 

A. Murata, Y. Baba, M. Watanabe, H. Shigaki, K. Miyake, T. Ishimoto, M. Iwatsuki, S. Iwagami, N. Yoshida, E. Oki, M. Morita, M. Nakao, and H. Baba. IGF2 DMR0 methylation, loss of imprinting, and patient prognosis in esophageal squamous cell carcinoma. Ann. Surg. Oncol. 21: 1166-1174, 2014.

 

Y. Baba, M. Watanabe, A. Murata, H. Shigaki, K. Miyake, T. Ishimoto, M. Iwatsuki, S. Iwagami, N. Yoshida, E. Oki, K. Sakamaki, M. Nakao, and H. Baba. LINE-1 hypomethylation, DNA copy number alterations, and CDK6 amplification in esophageal squamous cell carcinoma. Clin. Cancer Res. 20: 1114-1124, 2014.

 

N. Sasai, N. Saitoh, H. Saitoh, and M. Nakao. The transcriptional cofactor MCAF1/ATF7IP is involved in histone gene expression and cellular senescence. Plos One 87: e68478, 2013.

 

S. Hino, K. Nagaoka, and M. Nakao. Metabolism-epigenome crosstalk in physiology and diseases. J. Hum. Genet. (Reviews, Special Section on Epigenomics: biological understanding and clinical application) 58: 410-415, 2013.

 

S. Hino, A. Sakamoto, K. Nagaoka, K. Anan, Y. Wang, S. Mimasu, T. Umehara, S. Yokoyama, K. Kosai, and M. Nakao. FAD-dependent lysine demethylase LSD1 regulates cellular energy expenditure. Nature Commun. 3: 758, 2012.

 

A. Hirosue, K. Ishihara, K. Tokunaga, T. Watanabe, N. Saitoh, T. Chandra, M. Narita, M. Shinohara, and M. Nakao. Quantitative assessment of higher-order chromatin structure of the INK4/ARF locus in human senescent cells. Aging Cell 11: 553-556, 2012.

 

T. Watanabe, K. Ishihara, A. Hirosue, S. Watanabe, S. Hino, H. Ojima, Y. Kanai, Y. Sasaki, and M. Nakao. Higher-order chromatin regulation and differential gene expression in human tumor necrosis factor/lymphotoxin locus in hepatocellular carcinoma cells. Mol. Cell. Biol. 32: 1529-1541, 2012.

 

M. Taura, M.A. Suico, K. Koyama, K. Komatsu, R. Miyakita, C. Matsumoto, E. Kudo, R. Kariya, H. Goto, S. Kitajima, C. Takahashi, T. Shuto, M. Nakao, S. Okada, and H. Kai. Rb/E2F1 regulate innate immune receptor Toll-like receptor 3 in epithelial cells. Mol. Cell. Biol. 32: 1581-1590, 2012.

 

A. Fournier, N. Sasai, M. Nakao and P.A. Defossez. The role of methyl-binding proteins in chromatin organization and epigenome maintenance. Brief Funct. Genomics 11: 251-264, 2012.

N. Saitoh, C. Sakamoto, M. Hagiwara, L.T. Agredano-Moreno, L.F. Jimenez-Garcia, and M. Nakao. The distribution of phosphorylated SR proteins and alternative splicing are regulated by RANBP2. Mol. Biol. Cell 23: 1115-1128, 2012.

 

Y. Xi, S. Watanabe, Y. Hino, C. Sakamoto, Y. Nakatsu, S. Okada, and M. Nakao. Hmga1 is differentially expressed and mediates silencing of the Cd4/Cd8 loci in T cell lineages and leukemic cells. Cancer Sci. 103: 439-447, 2012.

 

S. Nagashima, T. Fukuda, Y. Kubota, A. Sugiura, M. Nakao, R. Inatome, and S. Yanagi. CRAG protects neuronal cells against cytotoxicity of expanded polyglutamine protein partially via c-Fos-dependent AP-1 activation. J. Biol. Chem. 286: 33879-33889, 2011.

 

N. Sasai, M. Nakao, and P-A. Defossez. Sequence-specific recognition of methylated DNA by human zinc-finger proteins. Nucleic Acids Res. 38: 5015-5022, 2010.

 

L-K. Chang, J-Y. Chuang, M. Nakao, S-T. Liu. MCAF1 and Synergistic activation of the transcription of Epstein-Barr virus lytic genes by Rta and Zta. Nucleic Acids Res. 38: 4687-4700, 2010.

 

J. Uwada, N. Tanaka, Y. Yamaguchi, Y. Uchimura, K. Shibahara, M. Nakao, and H. Saitoh. The p150 subunit of CAF-1 causes association of SUMO2/3 with the DNA replication foci. Biochem. Biophys. Res. Commun. 391: 407-413, 2010.

 

S. Akaboshi, S. Watanabe, Y. Hino, Y. Sekita, K. Araki, K. Yamamura, M. Oshima, T. Ito, H. Baba, and M. Nakao. HMGA1 is induced by Wnt/??catenin pathway and maintains cell proliferation in gastric cancer. Am. J. Pathol. 175: 1675-1685, 2009.

 

S. Tei, N. Saitoh, T. Funahara, S. Iida, Y. Nakatsu, Y. Kinoshita, H. Saya, and M. Nakao. Simian virus 40 large T antigen targets the microtubule-stabilizing protein TACC2. J. Cell Sci. 122: 3190-3198, 2009.

 

T. Mishiro, K. Ishihara, S. Hino, S. Tsutsumi, H. Aburatani, K. Shirahige, Y. Kinoshita, and M. Nakao. Architectural roles of multiple chromatin insulators at the human apolipoprotein gene cluster. EMBO J. 28: 1234-1245, 2009.

 

L. Liu, K. Ishihara, T. Ichimura, N. Fujita, S. Hino, S. Tomita, S. Watanabe, N. Saitoh, T. Ito, and M. Nakao. MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity.  J. Biol. Chem. 284: 5165-5174, 2009.

 

S. Watanabe, Y. Ueda, S. Akaboshi, Y. Hino, Y. Sekita, and M. Nakao. HMGA2 maintains oncogenic RAS-induced epithelial-mesenchymal transition in human pancreatic cancer cells. Am. J. Pathol. 174: 854-868, 2009.

 

T. Zhao, J. Yasunaga, M. Nakao, M. Fujii, and M. Matsuoka. Human T-cell leukemia virus type 1 bZIP factor selectively suppresses the classical pathway of NF-kB. Blood113: 2755-2764, 2009.

 

K. Kuwayama, K. Matsuzaki, Y. Mizobuchi, H. Mure, K. Kitazato, T. Kageji, M. Nakao, S. Nagahiro. Promyelocytic leukemia protein induces apoptosis due to caspase-8 activation via the repression of NF-kB activation in glioblastoma. Neuro-Oncol. 11:132-141, 2009.

 

T. Aoto, N. Saitoh, Y. Sakamoto, S. Watanabe, and M. Nakao. Polycomb group protein-associated chromatin is reproduced in post-mitotic G1 phase and required for S phase progression. J. Biol. Chem. 283: 18905-18915, 2008.

 

K.S. Wendt, K. Yoshida, T. Itoh, M. Bando, B. Koch, E. Schirghuber, S.Tsutsumi, G. Nagae, K. Ishihara, T. Mishiro, K. Yahata, F. Imamoto, H.Aburatani, M. Nakao, N. Imamoto, K. Maeshima, K. Shirahige, and J.M. Peters.Cohesin mediates transcriptional insulation by CCCTC-binding factor. Nature 451: 796-803, 2008.

 

Y. Ueda, S. Watanabe, S. Tei, N. Saitoh, J. Kuratsu, and M. Nakao. The high mobility group protein HMGA1 inhibits retinoblastoma protein-mediated cellular G0 arrest.Cancer Sci. 98: 1893-1901, 2007.

 

Y. Sakamoto, S. Watanabe, T. Ichimura, M. Kawasuji, H. Koseki, H. Baba, and M. Nakao. Overlapping roles of the methylated DNA binding protein MBD1 and polycomb group proteins in transcriptional repression of HoxA genes and heterochromatin foci formation. J. Biol. Chem. 282: 16391-16400, 2007.

 

S. Kobayakawa, K. Miike, M. Nakao, and K. Abe. Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells. Genes Cells 12: 447-460, 2007.

 

S. Tsuruzoe, K. Ishihara, Y. Uchimura, S. Watanabe, Y. Sekita, T. Aoto, H. Saitoh, Y. Yuasa, H. Niwa, M. Kawasuji, H. Baba, and M. Nakao. Inhibition of DNA binding of Sox2 by the SUMO conjugation. Biochem. Biophys. Res. Commun. 351: 920-926, 2006.

 

T. Aoto, N. Saitoh, T. Ichimura, H. Niwa, and M. Nakao. Nuclear and chromatin reorganization in the MHC-Oct3/4 locus at developmental phases of embryonic stem cell differentiation. Dev. Biol. 298: 345-367, 2006.

 

K. Ishihara, M. Oshimura, and M. Nakao. CTCF-dependent chromatin insulator is linked to epigenetic remodeling. Mol. Cell 23: 733-742, 2006.

 

MA. Suico, H. Nakamura, Z. Lu, H. Saitoh, T. Shuto, M. Nakao, and H. Kai. SUMO down-regulates the activity of Elf4/Myeloid Elf-1-like factor. Biochem. Biophys. Res. Commun. 348: 880-888, 2006.

 

Y. Uchimura, T. Ichimura, J. Uwada, T. Tachibana, S. Sugahara, M. Nakao, and H. Saitoh. Involvement of SUMO modification in MBD1- and MCAF1- mediated heterochromatin formation. J. Biol. Chem. 281: 23180-23190, 2006.

 

N. Saitoh, Y. Uchimura, T. Tachibana, S. Sugahara, H. Saitoh, and M. Nakao. In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies. Exp. Cell Res. 312: 1418-1430, 2006.

 

K. Ikeda, K. Iyama, N. Ishikawa, H. Egami, M. Nakao, Y. Sado, Y. Ninomiya, and H. Baba. Loss of expression of type IV collagen a5 and a6 chains in colorectal cancer associated with the hypermethylation of their promoter region. Am J. Pathol. 168: 856-865, 2006.

 

L-K. Chang, J-Y. Chuang, Y-R. Hong, T. Ichimura, M. Nakao, and S-T. Liu. Activation of Sp1-mediated transcription by Rta of Epstein-Barr virus via an interaction with MCAF1.Nucleic Acids Res. 33: 6528 –6539, 2005.

 

J. Fan, E. Kodama, Y. Koh, M. Nakao, and M. Matsuoka. Haloganated thymidine analogs restore the expression of silenced genes without demethylation. Cancer Res.65: 6927-6933, 2005.

 

Y. Arima, M. Nitta, S. Kuninaka, D. Zhang, T. Fujiwara, Y. Taya, M. Nakao, and H. Saya. Transcriptional blockade induces p53-dependent apoptosis associated with translocation of p53 to mitochondria. J. Biol. Chem. 280: 19166-19176, 2005.

 

T. Ichimura, S. Watanabe, Y. Sakamoto, T. Aoto, N. Fujita, and M. Nakao. Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins. J. Biol. Chem. 280: 13928-13935, 2005.

 

T. Kamikihara, T. Arima, K. Kato, T. Matsuda, H. Kato, T. Douchi, Y. Nagata, M. Nakao, and N. Wake. Epigenetic silencing of the imprinted gene ZAC by DNA methylation is an early event in the progression of human ovarian cancer. Int. J. Cancer  115: 690-700, 2005.

 

M. A. Suico, Z. Lu, T. Shuto, T. Koga, T. Uchikawa, H. Yoshida, K. Matsuzaki, M. Nakao, J-D. Li, and H. Kai. The regulation of human -Defensin 2 by the ETS transcription factor MEF is enhanced by PML protein. J. Pharmacol. Sci. 95: 466-470, 2004.

 

Y. Uchimura, M. Nakamura, K. Sugasawa, M. Nakao, and H. Saitoh. Overproduction of eukaryotic SUMO-1- and SUMO-2-conjugated proteins in Escherichia coli. Anal. Biochem. 331: 204-206, 2004.

 

M. Nakao, T. Minami, Y. Ueda, Y. Sakamoto, and T. Ichimura. Epigenetic system: a pathway to malignancies and a therapeutic target. Int. J. Hematol. 80: 103-107, 2004.

 

M. A. Suico, H. Yoshida, Y. Seki, T. Uchikawa, Z. Lu, T. Shuto, K. Matsuzaki, M. Nakao, J-D. Li, and H. Kai. The ETS transcription factor MEF up-regulates lysozyme transcription in epithelial cells through interaction with PML protein. J. Biol. Chem. 279: 19091-19098, 2004.

 

Y. Uchimura, M. Nakao, and H. Saitoh. Generation of SUMO-1 modified proteins in E. coli: towards understanding the biochemistry/structural biology of the SUMO-1 pathway. FEBS Lett. 564: 85-90, 2004.

 

S. Watanabe, T. Ichimura, N. Fujita, S. Tsuruzoe, I. Ohki, M. Shirakawa, M. Kawasuji. and M. Nakao. Methylated DNA-binding domain 1 and methylpurine-DNA glycosylase links transcriptional repression and DNA repair in chromatin. Proc. Natl. Acad. Sci. USA. 100: 12859-12864, 2003.

 

J. Yasuda, M. Nakao, Y. Kawaoka, and H. Shida. Nedd4 regulates Egress of Ebola virus-like particles from host cells. J. Virol. 77: 9987-9992, 2003.

 

N. Fujita, S. Watanabe, T. Ichimura, S. Tsuruzoe, Y. Shinkai, M. Tachibana, T. Chiba, and M. Nakao. Methyl-CpG binding domain 1 (MBD1) interacts with Suv39h1-HP1 heterochromatic complex for DNA methylation-based transcriptional repression. J. Biol. Chem. 278: 24132–24138, 2003.

 

S. Kudo, Y. Nomura, M. Segawa, N. Fujita, M. Nakao, C. Schanen, and M. Tamura. Heterogeneity in residual function of MeCP2 carrying missense mutations in the methyl-CpG-binding domain. J. Med. Genet. 40: 487-493, 2003.

 

K. Matsuzaki, T. Minami, M. Tojo, Y. Honda, Y. Uchimura, H. Saitoh, H. Yasuda, S. Nagahiro, H. Saya, and M. Nakao. Serum response factor is modulated by the SUMO-1 conjugation system. Biochem. Biophys. Res. Commun. 306: 32-38, 2003.

 

N. Fujita, S. Watanabe, T. Ichimura, Y. Ohkuma, T. Chiba, H. Saya, and M. Nakao. MCAF mediates MBD1-dependent transcriptional repression. Mol. Cell. Biol. 23: 2834-2843, 2003.

 

K. Matsuzaki, T. Minami, M. Tojo, Y. Honda, N. Saitoh, S. Nagahiro, H. Saya, and M. Nakao. PML-nuclear bodies are involved in cellular serum response. Genes Cells 8: 275-286, 2003.

 

M. Tojo, K. Matsuzaki, T. Minami, Y. Honda, H. Yasuda, T. Chiba, H. Saya, Y. Fujii-Kuriyama, and M. Nakao. The aryl hydrocarbon receptor nuclear transporter is modulated by the SUMO-1 conjugating system. J. Biol. Chem. 277: 46576-46585, 2002.

 

Y. Honda, M. Tojo, K. Matsuzaki, T. Anan, M. Matsumoto, M. Ando, H. Saya, and M. Nakao. Cooperation of HECT-domain ubiquitin ligase hHYD and DNA topoisomerase II-binding protein for DNA damage response. J. Biol. Chem. 277: 3599-3605, 2002.

 

J. Yasuda, E. Hunter, M. Nakao, and H. Shida. Functional involvement of a novel Nedd4-like ubiquitin ligase on retrovirus budding. EMBO Reports 3: 636-640, 2002.

 

K. Kagotani, H. Nabeshima, A. Kohda, M. Nakao, H. Taguchi, and K. Okumura. Visualization of transcription-dependent association of imprinted genes with the nuclear matrix. Exp. Cell Res. 274: 189-196, 2002.

 

S. Kudo, Y. Nomura, M. Segawa, M. Nakao, C. Schanen, M. Tamura. Functional characterization of MeCP2 mutations found in male patients with X-linked mental retardation. J. Med. Genet. 39: 132-136, 2002.

 

M. Nakao. Epigenetics: interactions of DNA methylation and chromatin. Gene 278: 25-31, 2001.

 

M. Nakao, S. Matsui, S. Yamamoto, K. Okumura, M. Shirakawa, and N. Fujita. Regulation of transcription and chromatin by methyl-CpG binding protein MBD1. Brain Dev. 23 Suppl 1: S174-176, 2001.

 

S. Kudo, Y. Nomura, M. Segawa, N. Fujita, M. Nakao, J. Dragich, C. Schanen, M. Tamura. Functional analyses of MeCP2 mutations associated with Rett syndrome using transient expression systems. Brain Dev. 23 Suppl 1: S165-173, 2001.

 

T. Sudo, Y. Ota, M. Nakao, Y. Takami, S. Takeda, and H. Saya. Activation of Cdh1-dependent APC is required for G1 cell cycle arrest and DNA damage-induced G2 checkpoint in vertebrate cells. EMBO J. 20: 6499-6508, 2001.

 

T. Takizawa, K. Nakashima, M. Namihira, W. Ochiai, A. Uemura, M. Yanagisawa, N. Fujita, M. Nakao, and T. Taga. DNA methylation is a critical cell-intrinsic determinant of astrocyte differentiation in the fetal brain. Dev. Cell 1: 749-758, 2001.

 

M. A. EL-Gazzar, K. Maeda, K. Kuwahara, H. Nomiyama, M. Nakao, and N. Sakaguchi. PU.1 is involved in the regulation of B lineage-associated and developmental stage-dependent expression of the germinal center-associated DNA-primase GANP. J. Biol. Chem. 276: 48000-48008, 2001.

 

T. Kino, H. Takeshima, M. Nakao, T. Nishi, K. Yamamoto, T. Kimura, Y. Saito, M. Kochi, J. Kuratsu, H. Saya, Y. Ushio. Identification of the cis-acting region in the NF2 gene promoter as a potential target for mutation and methylation-dependent silencing in schwannoma. Genes Cells 6: 441-454, 2001.

 

S. Takebayashi, M. Nakao, N. Fujita, T. Sado, M. Tanaka, H. Taguchi, and K. Okumura. 5-aza-2’-deoxycytidine induces histone hyperacetylation of mouse centromeric heterochromatin by a mechanism independent of DNA demethylation.Biochem. Biophys. Res. Commun. 288: 921-926, 2001.

 

T. Kaneko, T. Yamashima, Y. Tohma, M. Nomura, S. Imajoh-Ohmi, T.C. Saido, M.Nakao, H. Saya, and J. Yamashita. Calpain-dependent proteolysis of merlin occurs by oxidative stress in meningiomas. Cancer 92: 2662-2672, 2001.

 

I. Ohki, N. Shimotake, N. Fujita, J-G. Jee, T. Ikegami, M. Nakao, and M. Shirakawa. Solution structure of the methyl-CpG-binding domain of human MBD1 in complex with a methylated DNA. Cell 105: 487-497, 2001.

 

M. Meguro, A. Kashiwagi, K. Mitsuya, M. Nakao, I. Kondo, S. Saitoh, and M. Oshimura. A novel maternally expressed gene, ATP10C, encodes a putative aminophospholipid translocase associated with Angelman syndrome. Nature Genet. 28: 19-20, 2001.

 

M. Meguro, K. Mitsuya, N. Nomura, M. Kohda, A. Kashiwagi, R. Nishigaki, H. Yoshioka, M. Nakao, M. Oishi, and M. Oshimura. Large-scale evaluation of imprinting status in the Prader-Willi syndrome region: an imprinted direct repeat cluster resembling small nucleolar RNA genes. Hum. Mol. Genet. 10: 383-394, 2001.

 

N. Fujita, N. Shimotake, I. Ohki, T. Chiba, H. Saya, M. Shirakawa, and M. Nakao. Mechanism of transcriptional regulation by methyl-CpG binding protein MBD1. Mol. Cell. Biol. 20: 5107-5118, 2000.

 

M. Nogami, A. Kohda, H. Taguchi, M. Nakao, T. Ikemura, and K. Okumura. Relative locations of the centromere and imprinted SNRPN gene within chromosome 15 territories during the cell cycle in HL60 cells. J. Cell Sci. 113: 2157-2165, 2000. Y. Kimura, H. Saya, and M. Nakao. Calpain-dependent proteolysis of NF2 protein: The involvement in schwannomas and meningiomas. Neuropathol. 20: 153-160, 2000.

 

N. Mugita, Y. Honda, H. Nakamura, T. Fujiwara, K. Tanaka, S. Omura, N. Shimbara, M. Ogawa, H. Saya, and M. Nakao. The involvement of proteasome in myogenic differentiation of murine myocytes and human rhabdomyosarcoma cells. Int. J. Mol. Med. 3: 127-137, 1999.

 

I. Okamoto, Y. Kawano, H. Tsuiki, J. Sasaki, M. Nakao, M. Matsumoto, M. Suga, M. Ando, M. Nakajima, and H. Saya. CD44 cleavage induced by a membrane-associated metalloprotease plays a critical role in tumor cell migration. Oncogene 18: 1435-1446, 1999.

 

K. Yamamoto, H. Takeshima, K. Hamada, M. Nakao, T. Kino, T. Nishi, M. Kochi, J. Kuratsu, T. Yoshimura, and Y. Ushio. Cloning and functional characterization of the 5′-flanking region of the human monocyte chemoattractant protein-1 receptor (CCR2) gene. J. Biol. Chem. 274: 4646-4654, 1999.

 

Y. Nagata, T. Anan, T. Yoshida, T. Mizukami, Y. Taya, T. Fujiwara, H. Kato, H. Saya, and M. Nakao. The stabilization mechanism of mutant-type p53 by impaired ubiquitination: the loss of wild-type p53 function and the hsp90 association. Oncogene18: 6037-6049, 1999.

 

M. Nakao, Y. Kimura, and H. Saya. Emerging Therapeutic Targets in Meningiomas and Schwannomas: The Neurofibromatosis type 2 protein (Merlin). Emerging Therapeutic Targets (Ashley Publications Ltd.) 3: 335-364, 1999.

 

N. Fujita, S. Takebayashi, K. Okumura, S. Kudo, T. Chiba, H. Saya, and M. Nakao. Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms. Mol. Cell. Biol. 19: 6415-6426, 1999.

 

K. Ito, I. Okamoto, N. Araki, Y. Kawano, M. Nakao, S. Fujiyama, K. Tomita, T. Mimori, and H. Saya. Calcium influx triggers the sequential proteolysis of extracellular and cytoplasmic domains of E-cadherin, leading to loss of b-catenin from cell-cell contacts.Oncogene 18: 7080-7090, 1999.

I. Ohki, N. Shimotake, N. Fujita, M. Nakao, and M. Shirakawa. Solution structure of the methyl-CpG binding domain of the methylation-dependent transcriptional repressor MBD1. EMBO J. 18: 6653-6661, 1999.

 

H. Nakamura, M. Yoshida, H. Tsuiki, K. Ito, M. Ueno, M. Nakao, K. Oka, M. Tada, M. Kochi, J. Kuratsu, Y. Ushio, and H. Saya. Identification of a human homolog of the Drosophila neuralized gene within the 10q25.1 malignant astrocytoma deletion region.Oncogene 16: 1009-1019, 1998.

 

Y. Kimura, H. Koga, N. Araki, N. Mugita, N. Fujita, H. Takeshima, T. Nishi, T. Yamashima, T.C. Saido, T. Yamasaki, K. Moritake, H. Saya, and M. Nakao. The involvement of calpain-dependent proteolysis of the tumor suppressor NF2 (merlin) in schwannomas and meningiomas. Nature Med. 4: 915-922, 1998.

 

H. Koga, N. Araki, H. Takeshima, T. Nishi, T. Hirota, Y. Kimura, M. Nakao, and H. Saya. Impairment of cell adhesion by expression of the mutant neurofibromatosis type 2 (NF2) genes which miss exons in the ERM-homology domain. Oncogene 17: 801-810, 1998.

 

T. Anan, Y. Nagata, H. Koga, Y. Honda, N. Yabuki, C. Miyamoto, A. Kuwano, I. Matsuda, F. Endo, H. Saya, and M. Nakao. Human ubiquitin-protein ligase Nedd4: expression, subcellular localization, and selective interaction with ubiquitin-conjugating enzymes. Genes Cells 3 : 751-763, 1998.