研究会のご案内
リエゾンラボ研究会
発表内容

Title:
1. Ca2+ signal for initial left-right asymmetry development
2. Introduction to light-sheet microscopy for live imaging

Shigenori Nonaka
Laboratory for Spatiotemporal Regulations, Institute for Basic Biology (NIBB)

Abstract:
In mouse development, cilia-driven nodal flow determines left-right (L-R) asymmetric gene expression. The mechanism how the flow is converted into the asymmetric expression remains still controversial, while the importance of intracellular Ca2+ has been suggested. We found that node cells show transient increase of Ca2+ in apparently stochastic manner. Mapping of the frequency revealed that the Ca2+ increase was first symmetric, and become asymmetric as the flow develops. This tendency was disrupted in immotile cilia iv/iv and PKD2 mutant, a cation channel that is necessary for L-R determination. Here I will discuss possible mechanisms of the flow sensing.
As an additional topic, I’ll also talk about light-sheet microscopy, an emerging technology in recent years that has significant advantages for live specimen in comparison to widely used confocal microscopy, for its high penetration depth, low phototxicity/bleaching, and fast image acquisition rate. Here I will show some results on live imaging analysis of mouse gasrulation and high-speed visualization of moving Amoeba proteus .

References:
<L-R development>
Takao D, Nemoto T, Abe T, Kiyonari H, Kajiura-Kobayashi H, Shiratori H, Nonaka S. Asymmetric distribution of dynamic calcium signals in the node of mouse embryo during left-right axis formation. Developmental Biology 376:23-30, 2013

Nonaka S, Shiratori H, Saijoh Y, Hamada H. Determination of left-right patterning of the mouse embryo by artificial nodal flow. Nature . 418:96-99, 2002

<Light-sheet microscopy>
Takao D, Taniguchi A, Takeda T, Sonobe S, Nonaka S. High-speed imaging of amoeboid movements using light-sheet microscopy. PLoS One . 7:e50846, 2012